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Identification and localization of the membrane-associated, ATP-binding subunit of the oligopeptide permease of Salmonella typhimurium.

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  • المؤلفون: Gallagher MP;Gallagher MP; Pearce SR; Higgins CF
  • المصدر:
    European journal of biochemistry [Eur J Biochem] 1989 Mar 01; Vol. 180 (1), pp. 133-41.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • بيانات النشر:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • الموضوع:
      Bacterial Proteins*; Membrane Proteins/*metabolism ; Membrane Transport Proteins/*metabolism ; Receptors, Purinergic/*analysis ; Salmonella typhimurium/*enzymology; Binding Sites ; Cell Fractionation ; Cloning, Molecular ; Cytoplasm/metabolism ; Electrophoresis, Polyacrylamide Gel ; Hydrolysis ; Immune Sera/immunology ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/immunology ; Models, Molecular ; Plasmids ; Recombinant Proteins/metabolism ; Subcellular Fractions/enzymology
    • نبذة مختصرة :
      The OppF protein, a component of the oligopeptide permease of Salmonella typhimurium, is an ATP-binding protein and is believed to couple ATP hydrolysis to the transport process. This protein is an example of a large family of closely related proteins which couple ATP to a variety of different biological processes. The oppF gene has been cloned and sequenced. In order to identify and characterize its protein product we overproduced the protein from the cloned gene. Anti-OppF antibodies were raised against a synthetic peptide. Using these antibodies as a probe we identified OppF in wild-type and overproducing strains. Protease accessibility studies showed the protein to be a peripheral membrane protein located on the cytoplasmic side of the inner membrane. These findings have general implications for the organization and function of this class of prokaryotic and eukaryotic transport system.
    • الرقم المعرف:
      0 (Bacterial Proteins)
      0 (Immune Sera)
      0 (Membrane Proteins)
      0 (Membrane Transport Proteins)
      0 (Receptors, Purinergic)
      0 (Recombinant Proteins)
      39335-07-4 (oligopeptide permease, Bacteria)
    • الموضوع:
      Date Created: 19890301 Date Completed: 19890605 Latest Revision: 20190620
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1111/j.1432-1033.1989.tb14623.x
    • الرقم المعرف:
      2651120