Geometrical versus Random β-TCP Scaffolds: Exploring the Effects on Schwann Cell Growth and Behavior.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Biocompatible Materials/*pharmacology ; Calcium Phosphates/*pharmacology ; Cell Proliferation/*drug effects ; Schwann Cells/*drug effects; Animals ; Becaplermin ; Bone Regeneration/drug effects ; Gene Expression/drug effects ; Neovascularization, Physiologic/drug effects ; Neurotrophin 3/metabolism ; Porosity ; Proto-Oncogene Proteins c-sis ; Rats ; Rats, Inbred F344 ; Schwann Cells/metabolism ; Schwann Cells/physiology ; Tissue Scaffolds ; Vascular Endothelial Growth Factor A/metabolism

Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.

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R01 AR057837 United States AR NIAMS NIH HHS; R01 DE021468 United States DE NIDCR NIH HHS; R01AR057837 United States AR NIAMS NIH HHS; R01DE021468 United States DE NIDCR NIH HHS

0 (Biocompatible Materials)
0 (Calcium Phosphates)
0 (Neurotrophin 3)
0 (Proto-Oncogene Proteins c-sis)
0 (Vascular Endothelial Growth Factor A)
0 (beta-tricalcium phosphate)
1B56C968OA (Becaplermin)

Date Created: 20151008 Date Completed: 20160602 Latest Revision: 20181202

20250114

PMC4596809

10.1371/journal.pone.0139820

26444999