Structure and Sialyllactose Binding of the Carboxy-Terminal Head Domain of the Fibre from a Siadenovirus, Turkey Adenovirus 3.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Protein Structure, Tertiary*; Lactose/*analogs & derivatives ; Siadenovirus/*metabolism ; Sialic Acids/*chemistry ; Viral Proteins/*chemistry; Amino Acid Sequence ; Binding Sites/genetics ; Calorimetry/methods ; Carbohydrate Conformation ; Carbohydrate Sequence ; Crystallography, X-Ray ; Lactose/chemistry ; Lactose/metabolism ; Magnetic Resonance Spectroscopy ; Models, Molecular ; Molecular Sequence Data ; Mutation ; Polysaccharides/chemistry ; Polysaccharides/metabolism ; Protein Binding ; Siadenovirus/genetics ; Siadenovirus/pathogenicity ; Sialic Acids/metabolism ; Thermodynamics ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virulence/genetics

The virulent form of turkey adenovirus 3 (TAdV-3), also known as turkey hemorrhagic enteritis virus (THEV), is an economically important poultry pathogen, while the avirulent form is used as a vaccine. TAdV-3 belongs to the genus Siadenovirus. The carboxy-terminal region of its fibre does not have significant sequence similarity to any other adenovirus fibre heads of known structure. Two amino acid sequence differences between virulent and avirulent TAdV-3 map on the fibre head: where virulent TAdV-3 contains Ile354 and Thr376, avirulent TAdV-3 contains Met354 and Met376. We determined the crystal structures of the trimeric virulent and avirulent TAdV-3 fibre head domains at 2.2 Å resolution. Each monomer contains a beta-sandwich, which, surprisingly, resembles reovirus fibre head more than other adenovirus fibres, although the ABCJ-GHID topology is conserved in all. A beta-hairpin insertion in the C-strand of each trimer subunit embraces its neighbouring monomer. The avirulent and virulent TAdV-3 fibre heads are identical apart from the exact orientation of the beta-hairpin insertion. In vitro, sialyllactose was identified as a ligand by glycan microarray analysis, nuclear magnetic resonance spectroscopy, and crystallography. Its dissociation constant was measured to be in the mM range by isothermal titration calorimetry. The ligand binds to the side of the fibre head, involving amino acids Glu392, Thr419, Val420, Lys421, Asn422, and Gly423 binding to the sialic acid group. It binds slightly more strongly to the avirulent form. We propose that, in vivo, the TAdV-3 fibre may bind a sialic acid-containing cell surface component.

Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):271-81. (PMID: 21460445)
Anal Chem. 2012 Apr 3;84(7):3330-8. (PMID: 22390135)
Rev Med Virol. 2009 May;19(3):165-78. (PMID: 19367611)
Angew Chem Int Ed Engl. 1999 Jun 14;38(12 ):1784-1788. (PMID: 29711196)
Avian Dis. 1978 Jan-Mar;22(1):106-14. (PMID: 206247)
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002)
J Gen Virol. 2003 Nov;84(Pt 11):2895-908. (PMID: 14573794)
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8954-8. (PMID: 22611190)
J Virol Methods. 2010 Feb;163(2):262-8. (PMID: 19854219)
Infect Genet Evol. 2011 Jul;11(5):1058-65. (PMID: 21463713)
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. (PMID: 20057044)
Structure. 1994 Dec 15;2(12):1259-70. (PMID: 7704534)
J Virol. 2010 Oct;84(20):10558-68. (PMID: 20686025)
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1135-9. (PMID: 24100566)
Arch Virol. 1998;143(4):829-37. (PMID: 9638153)
J Biol Chem. 2006 Nov 3;281(44):33704-16. (PMID: 16923808)
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):386-94. (PMID: 21460457)
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):479-85. (PMID: 20383001)
J Am Chem Soc. 2009 Mar 25;131(11):3794-5. (PMID: 19292479)
Glycobiology. 2014 Jun;24(6):507-17. (PMID: 24658466)
PLoS One. 2014 Dec 08;9(12):e114373. (PMID: 25486282)
Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):30-42. (PMID: 15299723)
Avian Dis. 1977 Oct-Dec;21(4):557-65. (PMID: 415726)
Virus Res. 2008 Mar;132(1-2):132-9. (PMID: 18166240)
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):282-92. (PMID: 21460446)
PLoS Pathog. 2015 Feb 12;11(2):e1004657. (PMID: 25674795)
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42. (PMID: 21460441)
Mol Ther. 2005 Sep;12(3):384-93. (PMID: 15993650)
J Virol. 2003 Sep;77(17):9183-91. (PMID: 12915534)
Avian Dis. 1979 Jan-Mar;23(1):30-8. (PMID: 486006)
J Mol Biol. 2005 Nov 18;354(1):137-49. (PMID: 16236316)
Nat Protoc. 2008;3(7):1171-9. (PMID: 18600222)
Glycobiology. 2007 Aug;17(8):17C-23C. (PMID: 17483136)
J Gen Virol. 2007 Sep;88(Pt 9):2407-16. (PMID: 17698649)
Virus Res. 2006 Dec;122(1-2):144-53. (PMID: 16920215)
Virology. 1998 Sep 30;249(2):307-15. (PMID: 9791022)
Hum Gene Ther. 2004 Nov;15(11):1022-33. (PMID: 15610603)
Virology. 2008 Aug 15;378(1):169-76. (PMID: 18561970)
EMBO J. 2002 Jan 15;21(1-2):1-11. (PMID: 11782420)
J Virol. 2014 Oct;88(19):11304-14. (PMID: 25056898)
Expert Rev Vaccines. 2009 Jun;8(6):761-77. (PMID: 19485756)
Virology. 2012 Jan 5;422(1):144-50. (PMID: 22078165)
PLoS Pathog. 2008 Dec;4(12):e1000235. (PMID: 19079583)
J Vet Diagn Invest. 2009 Jul;21(4):415-26. (PMID: 19564489)
J Virol. 1998 Oct;72(10):7909-15. (PMID: 9733828)
J Biol Chem. 2007 Apr 13;282(15):11582-9. (PMID: 17303562)
Avian Pathol. 2009 Oct;38(5):413-7. (PMID: 19937528)
Vet Microbiol. 2014 Aug 6;172(1-2):35-43. (PMID: 24814929)
Mol Pharm. 2005 Sep-Oct;2(5):341-7. (PMID: 16196486)
J Biol Chem. 2006 May 19;281(20):14256-62. (PMID: 16549427)
Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9. (PMID: 20457744)
J Virol. 2004 Dec;78(23):13366-9. (PMID: 15542689)
Adv Protein Chem. 2006;73:97-124. (PMID: 17190612)
Science. 1999 Nov 19;286(5444):1579-83. (PMID: 10567268)
J Biol Chem. 2001 Mar 23;276(12):9009-15. (PMID: 11124261)
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67. (PMID: 21460454)
Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):915-9. (PMID: 22101817)
Avian Dis. 2009 Sep;53(3):370-5. (PMID: 19848074)
J Mol Biol. 2007 Sep 21;372(3):774-97. (PMID: 17681537)
Virus Res. 2002 Feb 26;83(1-2):189-95. (PMID: 11864751)
Virology. 1999 Sep 30;262(2):333-43. (PMID: 10502512)
Expert Opin Drug Deliv. 2008 Nov;5(11):1231-40. (PMID: 18976133)
Virology. 2002 Oct 10;302(1):33-43. (PMID: 12429514)
J Gen Virol. 2009 Aug;90(Pt 8):1978-85. (PMID: 19386786)
Adv Exp Med Biol. 2011;705:257-69. (PMID: 21618112)
Nature. 1999 Oct 28;401(6756):935-8. (PMID: 10553913)
J Gen Virol. 2000 Oct;81(Pt 10):2431-9. (PMID: 10993931)
J Virol. 2004 Jul;78(14):7727-36. (PMID: 15220447)
Acta Vet Hung. 2007 Mar;55(1):135-49. (PMID: 17385563)
J Mol Biol. 1995 Sep 29;252(4):379-85. (PMID: 7563058)
Viruses. 2012 May;4(5):847-77. (PMID: 22754652)
J Virol. 2000 Jan;74(1):42-8. (PMID: 10590089)
Methods Mol Biol. 2007;364:215-30. (PMID: 17172768)
Cell. 1993 Apr 23;73(2):309-19. (PMID: 8477447)
Angew Chem Int Ed Engl. 2003 Feb 24;42(8):864-90. (PMID: 12596167)
Nat Med. 2011 Jan;17(1):96-104. (PMID: 21151137)

0 (Polysaccharides)
0 (Sialic Acids)
0 (Viral Proteins)
3J7TAL60G3 (N-acetylneuraminoyllactose)
J2B2A4N98G (Lactose)

Date Created: 20150930 Date Completed: 20160531 Latest Revision: 20181113

20231215

PMC4587935

10.1371/journal.pone.0139339

26418008