Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Alternative Splicing*; Hippocampus/*metabolism ; Munc18 Proteins/*genetics ; Neurons/*metabolism; Amino Acid Sequence ; Animals ; Cells, Cultured ; Hippocampus/cytology ; Mice ; Molecular Sequence Data ; Munc18 Proteins/metabolism ; Neurons/physiology ; Rats ; Rats, Wistar ; Synapses/metabolism ; Synapses/physiology ; Synaptic Potentials

The munc18-1 gene encodes two splice-variants that vary at the C-terminus of the protein and are expressed at different levels in different regions of the adult mammalian brain. Here, we investigated the expression pattern of these splice variants within the brainstem and tested whether they are functionally different. Munc18-1a is expressed in specific nuclei of the brainstem including the LRN, VII and SOC, while Munc18-1b expression is relatively low/absent in these regions. Furthermore, Munc18-1a is the major splice variant in the Calyx of Held. Synaptic transmission was analyzed in autaptic hippocampal munc18-1 KO neurons re-expressing either Munc18-1a or Munc18-1b. The two splice variants supported synaptic transmission to a similar extent, but Munc18-1b was slightly more potent in sustaining synchronous release during high frequency stimulation. Our data suggest that alternative splicing of Munc18-1 support synaptic transmission to a similar extent, but could modulate presynaptic short-term plasticity.

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0 (Munc18 Proteins)

Date Created: 20150926 Date Completed: 20160617 Latest Revision: 20181113

20250114

PMC4583478

10.1371/journal.pone.0138950

26407320