Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Bacterial Proteins/*genetics ; Chromosome Mapping/*methods ; Genome, Bacterial/*genetics ; Trans-Activators/*genetics ; Transcriptional Activation/*genetics ; Xanthomonas/*genetics; Amino Acid Sequence ; Bacterial Proteins/chemistry ; Base Sequence ; High-Throughput Nucleotide Sequencing/methods ; Molecular Sequence Data ; Trans-Activators/chemistry

Xanthomonas TALE transcriptional activators act as virulence or avirulence factors by activating host disease susceptibility or resistance genes. Their specificity is determined by a tandem repeat domain. Some Xanthomonas pathogens contain 10-30 TALEs per strain. Although TALEs play critical roles in pathogenesis, their studies have so far been limited to a few examples, due to their highly repetitive gene structure and extreme similarity among different members, which constrict sequencing and assembling. To facilitate TALE studies, we developed an efficient and rapid pipeline for genome-wide cloning of tal genes as many as possible from a strain. Here, we report the pipeline and its use to identify all 18 tal genes from a newly isolated strain of the rice pathogen Xathomonas oryzae. Target prediction revealed a number of potential rice targets including several notable genes such as genes encoding SWEET, WRKY, Hen1, and BAK1 proteins, which provide candidates for further experimental functional analysis of the TALEs.

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0 (Bacterial Proteins)
0 (Trans-Activators)

Date Created: 20150815 Date Completed: 20160801 Latest Revision: 20181113

20240829

PMC4536657

10.1038/srep13162

26271455