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Endoscopic delivery of enteric neural stem cells to treat Hirschsprung disease.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 9432572 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2982 (Electronic) Linking ISSN: 13501925 NLM ISO Abbreviation: Neurogastroenterol Motil Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Osney Mead, Oxford, UK : Blackwell Scientific Publications, c1994-
    • الموضوع:
      Colonoscopy/*methods ; Enteric Nervous System/*cytology ; Hirschsprung Disease/*therapy ; Neural Stem Cells/*transplantation; Animals ; Disease Models, Animal ; Mice ; Mice, 129 Strain ; Mice, Inbred C57BL ; Mice, Transgenic ; Receptor, Endothelin B
    • نبذة مختصرة :
      Background: Transplantation of enteric neural stem cells (ENSC) holds promise as a potential therapy for enteric neuropathies, including Hirschsprung disease. Delivery of transplantable cells via laparotomy has been described, but we propose a novel, minimally invasive endoscopic method of cell delivery.
      Methods: Enteric neural stem cells for transplantation were cultured from dissociated gut of postnatal donor mice. Twelve recipient mice, including Ednrb(-/-) mice with distal colonic aganglionosis, underwent colonoscopic injection of ENSC under direct vision using a 30-gauge Hamilton needle passed through a rigid cystoureteroscope. Cell engraftment, survival, and neuroglial differentiation were studied 1-4 weeks after the procedure.
      Key Results: All recipient mice tolerated the procedure without complications and survived to sacrifice. Transplanted cells were found within the colonic wall in 9 of 12 recipient mice with differentiation into enteric neurons and glia.
      Conclusions & Inferences: Endoscopic injection of ENSC is a safe and reliable method for cell delivery, and can be used to deliver a large number of cells to a specific area of disease. This minimally invasive endoscopic approach may prove beneficial to future human applications of cell therapy for neurointestinal disease.
      (© 2015 John Wiley & Sons Ltd.)
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    • Grant Information:
      R01 DK080914 United States DK NIDDK NIH HHS; R01 DK103785 United States DK NIDDK NIH HHS; DK080914 United States DK NIDDK NIH HHS
    • Contributed Indexing:
      Keywords: Hirschsprung disease; cell therapy; endoscopy; enteric neural stem cells; enteric neuropathies
    • الرقم المعرف:
      0 (EDNRB protein, mouse)
      0 (Receptor, Endothelin B)
    • الموضوع:
      Date Created: 20150721 Date Completed: 20160706 Latest Revision: 20181113
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC4600089
    • الرقم المعرف:
      10.1111/nmo.12635
    • الرقم المعرف:
      26190543