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Bacterial Exposures and Associations with Atopy and Asthma in Children.

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  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Asthma/*immunology ; Bacteria/*immunology ; Environmental Exposure/*adverse effects ; Hypersensitivity, Immediate/*immunology; Age Factors ; Air Pollution, Indoor/adverse effects ; Asthma/epidemiology ; Asthma/microbiology ; Bacteria/classification ; Bacteria/genetics ; Biodiversity ; Child ; Female ; Humans ; Hypersensitivity, Immediate/epidemiology ; Hypersensitivity, Immediate/microbiology ; Male ; Molecular Typing ; Qualitative Research ; Risk Factors
    • نبذة مختصرة :
      Background: The increase in prevalence of asthma and atopic diseases in Western countries has been linked to aspects of microbial exposure patterns of people. It remains unclear which microbial aspects contribute to the protective farm effect.
      Objective: The objective of this study was to identify bacterial groups associated with prevalence of asthma and atopy, and to quantify indoor exposure to some of these bacterial groups.
      Methods: A DNA fingerprinting technique, denaturing gradient gel electrophoresis (DGGE), was applied to mattress dust samples of farm children and control children in the context of the GABRIEL Advanced study. Associations between signals in DGGE and atopy, asthma and other allergic health outcomes were analyzed. Quantitative DNA based assays (qPCR) for four bacterial groups were applied on the dust samples to seek quantitative confirmation of associations indicated in DNA fingerprinting.
      Results: Several statistically significant associations between individual bacterial signals and also bacterial diversity in DGGE and health outcomes in children were observed. The majority of these associations showed inverse relationships with atopy, less so with asthma. Also, in a subsequent confirmation study using a quantitative method (qPCR), higher mattress levels of specifically targeted bacterial groups - Mycobacterium spp., Bifidobacteriaceae spp. and two different clusters of Clostridium spp. - were associated with a lower prevalence of atopy.
      Conclusion: DNA fingerprinting proved useful in identifying bacterial signals that were associated with atopy in particular. These findings were quantitatively confirmed for selected bacterial groups with a second method. High correlations between the different bacterial exposures impede a clear attribution of protective effects to one specific bacterial group. More diverse bacterial flora in mattress dust may link to microbial exposure patterns that protect against development of atopic diseases.
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    • الموضوع:
      Date Created: 20150630 Date Completed: 20160406 Latest Revision: 20220330
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC4488145
    • الرقم المعرف:
      10.1371/journal.pone.0131594
    • الرقم المعرف:
      26121165