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NEUROSCIENCE. Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics.

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  • معلومة اضافية
    • المصدر:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
    • بيانات النشر:
      Publication: : Washington, DC : American Association for the Advancement of Science
      Original Publication: New York, N.Y. : [s.n.] 1880-
    • الموضوع:
      Chloride Channels/*physiology ; Cryptophyta/*metabolism ; Membrane Potentials/*radiation effects ; Neurons/*radiation effects ; Optogenetics/*methods ; Rhodopsins, Microbial/*physiology; Amino Acid Sequence ; Chloride Channels/classification ; Chloride Channels/genetics ; Cryptophyta/genetics ; HEK293 Cells ; Humans ; Ion Channel Gating ; Light ; Membrane Potentials/physiology ; Molecular Sequence Data ; Neural Inhibition ; Neurons/physiology ; Photic Stimulation ; Phylogeny ; Rhodopsins, Microbial/classification ; Rhodopsins, Microbial/genetics ; Transfection
    • نبذة مختصرة :
      Light-gated rhodopsin cation channels from chlorophyte algae have transformed neuroscience research through their use as membrane-depolarizing optogenetic tools for targeted photoactivation of neuron firing. Photosuppression of neuronal action potentials has been limited by the lack of equally efficient tools for membrane hyperpolarization. We describe anion channel rhodopsins (ACRs), a family of light-gated anion channels from cryptophyte algae that provide highly sensitive and efficient membrane hyperpolarization and neuronal silencing through light-gated chloride conduction. ACRs strictly conducted anions, completely excluding protons and larger cations, and hyperpolarized the membrane of cultured animal cells with much faster kinetics at less than one-thousandth of the light intensity required by the most efficient currently available optogenetic proteins. Natural ACRs provide optogenetic inhibition tools with unprecedented light sensitivity and temporal precision.
      (Copyright © 2015, American Association for the Advancement of Science.)
    • Comments:
      Comment in: Science. 2015 Aug 7;349(6248):590-1. (PMID: 26250674)
      Comment in: Nat Methods. 2015 Sep;12(9):806-7. (PMID: 26554088)
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    • Grant Information:
      R21 MH098288 United States MH NIMH NIH HHS; R21MH098288 United States MH NIMH NIH HHS; R01 GM027750 United States GM NIGMS NIH HHS; S10RR022531 United States RR NCRR NIH HHS; S10 RR022531 United States RR NCRR NIH HHS; R01GM027750 United States GM NIGMS NIH HHS
    • الرقم المعرف:
      0 (Chloride Channels)
      0 (Rhodopsins, Microbial)
    • الموضوع:
      Date Created: 20150627 Date Completed: 20150903 Latest Revision: 20220309
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC4764398
    • الرقم المعرف:
      10.1126/science.aaa7484
    • الرقم المعرف:
      26113638