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Determination of Mother Centriole Maturation in CPAP-Depleted Cells Using the Ninein Antibody.

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  • المؤلفون: Lee M;Lee M; Rhee K; Rhee K
  • المصدر:
    Endocrinology and metabolism (Seoul, Korea) [Endocrinol Metab (Seoul)] 2015 Mar 27; Vol. 30 (1), pp. 53-7. Date of Electronic Publication: 2014 Jul 02.
  • نوع النشر :
    Journal Article
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Korean Endocrine Society Country of Publication: Korea (South) NLM ID: 101554139 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2093-596X (Print) Linking ISSN: 2093596X NLM ISO Abbreviation: Endocrinol Metab (Seoul) Subsets: PubMed not MEDLINE
    • بيانات النشر:
      Original Publication: Seoul : Korean Endocrine Society, [2010]-
    • نبذة مختصرة :
      Background: Mutations in centrosomal protein genes have been identified in a number of genetic diseases in brain development, including microcephaly. Centrosomal P4.1-associated protein (CPAP) is one of the causal genes implicated in primary microcephaly. We previously proposed that CPAP is essential for mother centriole maturation during mitosis.
      Methods: We immunostained CPAP-depleted cells using the ninein antibody, which selectively detects subdistal appendages in mature mother centrioles.
      Results: Ninein signals were significantly impaired in CPAP-depleted cells.
      Conclusion: The results suggest that CPAP is required for mother centriole maturation in mammalian cells. The selective absence of centriolar appendages in young mother centrioles may be responsible for asymmetric spindle pole formation in CPAP-depleted cells.
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    • Contributed Indexing:
      Keywords: Cell cycle; Centrioles; Centrosomal P4.1-associated protein; Centrosome; Microcephaly; Ninein
    • الموضوع:
      Date Created: 20150402 Date Completed: 20150401 Latest Revision: 20181113
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC4384682
    • الرقم المعرف:
      10.3803/EnM.2015.30.1.53
    • الرقم المعرف:
      25827458