Age-related expression of Neurexin1 and Neuroligin3 is correlated with presynaptic density in the cerebral cortex and hippocampus of male mice.

Publisher: Springer Country of Publication: Netherlands NLM ID: 101250497 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1574-4647 (Electronic) Linking ISSN: 01619152 NLM ISO Abbreviation: Age (Dordr) Subsets: MEDLINE

Original Publication: Dordrecht, The Netherlands ; Hingham, MA : Springer, c2005-

Aging/*physiology ; Cell Adhesion Molecules, Neuronal/*metabolism ; Cerebral Cortex/*metabolism ; Hippocampus/*metabolism ; Membrane Proteins/*metabolism ; Nerve Tissue Proteins/*metabolism ; Neural Cell Adhesion Molecules/*metabolism ; Neuronal Plasticity/*physiology ; Presynaptic Terminals/*metabolism; Animals ; Calcium-Binding Proteins ; Cognitive Aging/physiology ; Immunoblotting ; Male ; Mice, Inbred Strains ; Reverse Transcriptase Polymerase Chain Reaction ; Synaptophysin/physiology

Neurexin1 (Nrxn1) and Neuroligin3 (Nlgn3) are cell adhesion proteins, which play an important role in synaptic plasticity that declines with advancing age. However, the expression of these proteins during aging has not been analyzed. In the present study, we have examined the age-related changes in the expression of these proteins in cerebral cortex and hippocampus of 10-, 30-, 50-, and 80-week-old male mice. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis indicated that messenger RNA (mRNA) level of Nrxn1 and Nlgn3 significantly increased from 10 to 30 weeks and then decreased at 50 weeks in both the regions. However, in 80-week-old mice, Nrxn1 and Nlgn3 were further downregulated in cerebral cortex while Nrxn1 was downregulated and Nlgn3 was upregulated in hippocampus. These findings were corroborated by immunoblotting and immunofluorescence results. When the expression of Nrxn1 and Nlgn3 was correlated with presynaptic density marker synaptophysin, it was found that synaptophysin protein expression in cerebral cortex was high at 10 weeks and decreased gradually up to 80 weeks, whereas in hippocampus, it decreased until 50 weeks and then increased remarkably at 80 weeks. Furthermore, Pearson's correlation analysis showed that synaptophysin had a strong relation with Nrxn1 and Nlgn3 in cerebral cortex and with Nlgn3 in hippocampus. Thus, these findings showed that Nrxn1 and Nlgn3 are differentially expressed in cerebral cortex and hippocampus which might be responsible for alterations in synaptic plasticity during aging.

Erratum in: Geroscience. 2019 Oct;41(5):709-710. (PMID: 31485888)

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0 (Calcium-Binding Proteins)
0 (Cell Adhesion Molecules, Neuronal)
0 (Membrane Proteins)
0 (Nerve Tissue Proteins)
0 (Neural Cell Adhesion Molecules)
0 (Nrxn1 protein, mouse)
0 (SYP protein, human)
0 (Synaptophysin)
0 (neuroligin 3)

Date Created: 20150220 Date Completed: 20160211 Latest Revision: 20201209

20231215

PMC4332888

10.1007/s11357-015-9752-6

25693924