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Chronic intraocular pressure elevation impairs autoregulatory capacity in streptozotocin-induced diabetic rat retina.

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  • المؤلفون: Wong VH;Wong VH; Armitage JA; He Z; Hui F; Vingrys AJ; Bui BV
  • المصدر:
    Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists) [Ophthalmic Physiol Opt] 2015 Mar; Vol. 35 (2), pp. 125-34. Date of Electronic Publication: 2014 Dec 20.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Blackwell Publishers Country of Publication: England NLM ID: 8208839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1475-1313 (Electronic) Linking ISSN: 02755408 NLM ISO Abbreviation: Ophthalmic Physiol Opt Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2002- : Oxford : Blackwell Publishers
      Original Publication: Oxford ; New York : Pergamon Press, c1981-
    • الموضوع:
      Diabetes Mellitus, Experimental/*physiopathology ; Eye/*blood supply ; Intraocular Pressure/*physiology ; Ocular Hypertension/*physiopathology ; Regional Blood Flow/*physiology ; Retina/*physiology; Analysis of Variance ; Animals ; Electroretinography ; Male ; Photoreceptor Cells, Vertebrate/physiology ; Rats ; Rats, Long-Evans ; Retinal Ganglion Cells/physiology ; Sensory Thresholds/physiology ; Streptozocin/pharmacology ; Stress, Physiological/physiology
    • نبذة مختصرة :
      Purpose: To assess ocular blood flow responses to acute IOP stress following 4 weeks of chronic IOP elevation in streptozotocin (STZ)-induced diabetic and control rats. We hypothesise that chronic IOP elevation for 4 weeks will further impair blood flow regulation in STZ-induced diabetic rats eyes.
      Methods: Two weeks following citrate buffer or STZ-injections chronic IOP elevation was induced in Long Evans rats via fortnightly intracameral injections of microspheres (15 μm) suspended in 5% polyethylene glycol. IOP was monitored daily. Electroretinography (ERG, -6.79-2.07 log cd s m(-2) ) was undertaken at Week 4 to compare photoreceptor (RmPIII ), ON-bipolar cell (Vmax ) and ganglion cell dominant ERG [scotopic threshold response (STR)] components. 4 weeks post-chronic IOP induction, ocular blood flow (laser Doppler flowmetry) was measured in response to acute IOP challenge (10-100 mmHg, in 5 mmHg steps, each 3 min).
      Results: Four weeks of chronic IOP (mean ± S.E.M., citrate: 24.0 ± 0.3 to 30.7 ± 1.3 and STZ-diabetes: 24.2 ± 0.2 to 31.1 ± 1.2 mmHg) was associated with reduced photoreceptor amplitude in both groups (-25.3 ± 2.2% and -17.2 ± 3.0%, respectively). STZ-diabetic eyes showed reduced photoreceptor sensitivity (citrate: 0.5 ± 1.8%, STZ-diabetic: -8.1 ± 2.4%). Paradoxically ON-bipolar cell sensitivity was increased, particularly in citrate control eyes (citrate: 166.8 ± 25.9%, STZ-diabetic: 64.8 ± 18.7%). The ganglion cell dominant STR was not significantly reduced in STZ-diabetic rats. Using acute IOP elevation to probe autoregulation, we show that STZ-diabetes impaired autoregulation compared with citrate control animals. The combination of STZ-diabetes and chronic IOP elevation further impaired autoregulation.
      Conclusions: STZ-diabetes and chronic IOP elevation appear to be additive risk factors for impairment of ocular blood flow autoregulation.
      (© 2014 The Authors Ophthalmic & Physiological Optics © 2014 The College of Optometrists.)
    • Contributed Indexing:
      Keywords: blood flow; diabetes; electroretinogram; intraocular pressure; rat; retina
    • الرقم المعرف:
      5W494URQ81 (Streptozocin)
    • الموضوع:
      Date Created: 20141223 Date Completed: 20151117 Latest Revision: 20150312
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1111/opo.12174
    • الرقم المعرف:
      25529024