Functional repression of PtSND2 represses growth and development by disturbing auxin biosynthesis, transport and signaling in transgenic poplar.

Publisher: Oxford University Press Country of Publication: Canada NLM ID: 100955338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1758-4469 (Electronic) Linking ISSN: 0829318X NLM ISO Abbreviation: Tree Physiol Subsets: MEDLINE

Publication: Oxford : Oxford University Press
Original Publication: Victoria, [B.C.] : Heron Pub., c1986-

Gene Expression Regulation, Plant*; Indoleacetic Acids/*metabolism ; Plant Proteins/*genetics ; Plants, Genetically Modified/*genetics ; Populus/*genetics ; Transcription Factors/*genetics; Biological Transport/genetics ; Plant Leaves/genetics ; Plant Leaves/growth & development ; Plant Roots/genetics ; Plant Roots/growth & development ; Plant Stems/genetics ; Plant Stems/growth & development ; Plants, Genetically Modified/growth & development ; Populus/growth & development ; Signal Transduction/genetics

Using chimeric repressor silencing technology, we previously reported that functional repression of PtSND2 severely arrested wood formation in transgenic poplar (Populus). Here, we provide further evidence that auxin biosynthesis, transport and signaling were disturbed in these transgenic plants, leading to pleiotropic defects in their growth patterns, including inhibited leaf enlargement and vascular tissue development in the leaf central vein, suppressed cambial growth and fiber elongation in the stem, and arrested growth in the root system. Two transgenic lines, which displayed the most remarkable phenotypic deviation from the wild-type, were selected for detailed studies. In both transgenic lines, expression of genes for auxin biosynthesis, transport and signaling was down-regulated, and indole-3-acetic acid distribution was severely disturbed in the apical buds, leaves, stems and roots of field-grown transgenic plants. Transient transcription dual-luciferase assays of ProPtTYDC2::LUC, ProPttLAX2::LUC and ProPoptrIAA20.2::LUC in poplar protoplasts revealed that expression of auxin-related genes might be regulated by PtSND2 at the transcriptional level. All these results indicate that functional repression of PtSND2 altered auxin biosynthesis, transport and signaling, and thereby disturbed the normal growth and development of transgenic plants.
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Keywords: PtSND2; chimeric repressor; indole-3-acetic acid; transgenic plant

0 (Indoleacetic Acids)
0 (Plant Proteins)
0 (Transcription Factors)
6U1S09C61L (indoleacetic acid)

Date Created: 20141218 Date Completed: 20150918 Latest Revision: 20150124

20221213

10.1093/treephys/tpu100

25516528