Overexpression of EaDREB2 and pyramiding of EaDREB2 with the pea DNA helicase gene (PDH45) enhance drought and salinity tolerance in sugarcane (Saccharum spp. hybrid).

Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer, 1981-

DNA Helicases/*genetics ; Pisum sativum/*enzymology ; Saccharum/*physiology ; Transcription Factors/*genetics; Base Sequence ; Chlorophyll/metabolism ; DNA Helicases/metabolism ; Gene Expression ; Gene Expression Regulation, Plant ; Molecular Sequence Data ; Pisum sativum/genetics ; Plant Leaves/drug effects ; Plant Leaves/genetics ; Plant Leaves/physiology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Transpiration/physiology ; Plants, Genetically Modified ; Promoter Regions, Genetic/genetics ; Saccharum/drug effects ; Saccharum/genetics ; Salinity ; Salt Tolerance ; Sequence Analysis, DNA ; Sodium Chloride/pharmacology ; Stress, Physiological ; Transcription Factors/metabolism

Key Message: EaDREB2 overexpressed in sugarcane enhanced tolerance to drought and salinity. When co-transformed with plant DNA helicase gene, DREB2 showed greater level of salinity tolerance than in single-gene transgenics. Drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and can potentially cause up to 50 % yield loss. DREB proteins play a vital regulatory role in abiotic stress tolerance in plants. We previously reported that expression of EaDREB2 is enhanced by drought stress in Erianthus arundinaceus. In this study, we have isolated the DREB2 gene from E. arundinaceus, transformed one of the most popular sugarcane variety Co 86032 in tropical India with EaDREB2 through Agrobacterium-mediated transformation, pyramided the EaDREB2 gene with the gene coding for PDH45 driven by Port Ubi 2.3 promoter through particle bombardment and evaluated the V1 transgenics for soil deficit moisture and salinity stresses. Soil moisture stress was imposed at the tillering phase by withholding irrigation. Physiological, molecular and morphological parameters were used to assess drought tolerance. Salinity tolerance was assessed through leaf disc senescence and bud sprout assays under salinity stress. Our results indicate that overexpression of EaDREB2 in sugarcane enhances drought and salinity tolerance to a greater extent than the untransformed control plants. This is the first report of the co-transformation of EaDREB2 and PDH45 which shows higher salinity tolerance but lower drought tolerance than EaDREB2 alone. The present study seems to suggest that, for combining drought and salinity tolerance together, co-transformation is a better approach.

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GENBANK KJ670161

0 (Plant Proteins)
0 (Transcription Factors)
1406-65-1 (Chlorophyll)
451W47IQ8X (Sodium Chloride)
EC 3.6.4.- (DNA Helicases)

Date Created: 20141206 Date Completed: 20151020 Latest Revision: 20240109

20240109

10.1007/s00299-014-1704-6

25477204