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Hybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank).

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Genome* ; Nucleic Acid Hybridization*; Polymerase Chain Reaction/*methods; Animals ; DNA, Bacterial/genetics ; Escherichia coli/genetics ; Mice ; Molecular Sequence Data ; Rats
    • نبذة مختصرة :
      Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. We demonstrate that during capture, the bait-hybridized library molecules add additional flanking library sequences iteratively, such that baits limited to targeting relatively short regions (e.g. few hundred nucleotides) can result in enrichment across entire mitochondrial and bacterial genomes. Our findings suggest that some of the off-target sequences derived in capture experiments are non-randomly enriched, and that CapFlank will facilitate targeted enrichment of large contiguous sequences with minimal prior target sequence information.
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    • Grant Information:
      R01 GM092706 United States GM NIGMS NIH HHS; R01GM092706 United States GM NIGMS NIH HHS
    • Molecular Sequence:
      GENBANK KJ530551; KJ530552; KJ530553; KJ530554; KJ530555; KJ530556; KJ530557; KJ530558; KJ530559; KJ530560; KJ530561; KJ530562; KJ530563; KJ530564; KJ530565
    • الرقم المعرف:
      0 (DNA, Bacterial)
    • الموضوع:
      Date Created: 20141003 Date Completed: 20151207 Latest Revision: 20211021
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC4183570
    • الرقم المعرف:
      10.1371/journal.pone.0109101
    • الرقم المعرف:
      25275614