Pepper mildew resistance locus O interacts with pepper calmodulin and suppresses Xanthomonas AvrBsT-triggered cell death and defense responses.

Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag [etc.]

Calmodulin/*metabolism ; Capsicum/*genetics ; Plant Diseases/*immunology ; Plant Proteins/*metabolism ; Xanthomonas campestris/*physiology; Calmodulin/genetics ; Capsicum/immunology ; Cell Death ; Disease Resistance ; Gene Expression ; Gene Silencing ; Genes, Reporter ; Genetic Loci/genetics ; Onions/cytology ; Onions/genetics ; Plant Diseases/microbiology ; Plant Leaves/genetics ; Plant Leaves/immunology ; Plant Leaves/microbiology ; Plant Proteins/genetics ; Recombinant Fusion Proteins ; Nicotiana/cytology ; Nicotiana/genetics ; Nicotiana/immunology ; Two-Hybrid System Techniques

Main Conclusion: Pepper CaMLO2 specifically interacts with CaCaM1 and translocates cytoplasmic CaCaM1 to the plasma membrane, leading to the suppression of Xanthomonas AvrBsT-triggered Ca (2+) influx, hypersensitive cell death and defense responses. Pathogen-induced cell death is closely linked with disease susceptibility and resistance in plants. Pepper (Capsicum annuum) mildew resistance locus O (CaMLO2) and calmodulin (CaCaM1) genes are required for disease-associated cell death and hypersensitive cell death, respectively. Here, we demonstrate that pathogen-responsive CaMLO2 interacts with CaCaM1 in yeast and in planta. Bimolecular fluorescence complementation and co-immunoprecipitation analyses confirm a specific interaction between CaMLO2 and CaCaM1 at the plasma membrane (PM) in plant cells. Subcellular localization analyses of CaCaM1 fused to green fluorescent protein reveals that treatment with Ca(2+) and co-expression with CaMLO2 induce translocation of cytosolic CaCaM1 to the PM where CaMLO2 is localized. Transient CaMLO2 expression negatively regulates CaCaM1 accumulation in Nicotiana benthamiana. Xanthomonas avrBsT-triggered Ca(2+) influx and hypersensitive cell death are disrupted by CaCaM1 and/or CaMLO2 expression. CaMLO2 silencing in pepper significantly enhances reactive oxygen species burst, cell death, and resistance responses to Xanthomonas campestris pv. vesicatoria Ds1 and Ds1 (avrBsT), which is accompanied by enhanced induction of CaCaM1, CaPR1 (PR-1), and CaPO2 (peroxidase). These results suggest that CaMLO2 interacts with CaCaM1 and suppresses AvrBsT-triggered cell death and defense responses.

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0 (Calmodulin)
0 (Plant Proteins)
0 (Recombinant Fusion Proteins)

Date Created: 20140731 Date Completed: 20151111 Latest Revision: 20231213

20231215

10.1007/s00425-014-2134-y

25074588