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Transgenic rice as bioreactor for production of the Candida antarctica lipase B.

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  • المؤلفون: Yang Y;Yang Y; Wang D; Zhang X; Fang J; Shen Z; Lin C
  • المصدر:
    Plant biotechnology journal [Plant Biotechnol J] 2014 Sep; Vol. 12 (7), pp. 963-70. Date of Electronic Publication: 2014 May 23.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists Country of Publication: England NLM ID: 101201889 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1467-7652 (Electronic) Linking ISSN: 14677644 NLM ISO Abbreviation: Plant Biotechnol J Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2014- : Oxford Wiley on behalf of the Society for Experimental Biology, Association of Applied Biologists
      Original Publication: [Oxford] : Blackwell Pub., c2003-
    • الموضوع:
      Bioreactors*; Fungal Proteins/*biosynthesis ; Lipase/*biosynthesis ; Oryza/*genetics ; Plants, Genetically Modified/*metabolism; Biofuels ; Fermentation ; Fungal Proteins/genetics ; Fungal Proteins/isolation & purification ; Lipase/genetics ; Lipase/isolation & purification ; Promoter Regions, Genetic ; Transformation, Genetic
    • نبذة مختصرة :
      Candida antarctica lipase B (CALB) is a versatile biocatalyst used for a wide range of biotransformation. Methods for low cost production of this enzyme are highly desirable. Here, we report a mass production method of CALB using transgenic rice seeds as the bioreactor. The transgenic rice transformed with the CALB gene under the control of the promoter of the rice seed storage protein GT1 was found to have accumulated a large quantity of CALB in seeds. The transgenic line with the highest lipolytic activity reached to 85 units per gram of dry seeds. One unit is defined as the amount of lipase necessary to liberate 1 μmol p-nitrophenol from p-nitrophenyl butyrate in 1 min. The rice recombinant lipase (rOsCALB) from this line represents 40% of the total soluble proteins in the crude seed extracts. The enzyme purified from the rice seeds had an optimal temperature of 40 °C, and optimal pH of 8.5, similar to that of the fermentation products. Test of its conversion ability as a biocatalyst for biodiesel production suggested that rOsCALB is functionally identical to the fermentation products in its industrial application.
      (© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.)
    • Contributed Indexing:
      Keywords: Candida antarctica lipase B; agronomic traits; enzyme activity; esterification; purification; transgenic rice
    • الرقم المعرف:
      0 (Biofuels)
      0 (Fungal Proteins)
      EC 3.1.1.3 (Lipase)
      EC 3.1.1.3 (lipase B, Candida antarctica)
    • الموضوع:
      Date Created: 20140524 Date Completed: 20150423 Latest Revision: 20161125
    • الموضوع:
      20231215
    • الرقم المعرف:
      10.1111/pbi.12204
    • الرقم المعرف:
      24852039