Cephalopod eye evolution was modulated by the acquisition of Pax-6 splicing variants.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Alternative Splicing*; Cephalopoda/*genetics ; Eye/*metabolism ; Eye Proteins/*genetics ; Homeodomain Proteins/*genetics ; Paired Box Transcription Factors/*genetics ; Repressor Proteins/*genetics; Amino Acid Sequence ; Animals ; Base Sequence ; Cephalopoda/embryology ; Exons ; Eye/embryology ; Eye Proteins/chemistry ; Gene Expression Regulation, Developmental ; Homeodomain Proteins/chemistry ; Introns ; Molecular Sequence Data ; PAX6 Transcription Factor ; Paired Box Transcription Factors/chemistry ; Repressor Proteins/chemistry ; Sequence Alignment

Previous studies have reported that the developmental processes of vertebrate eyes are controlled by four Pax-6 splicing variants, each modulating different downstream genes, whereas those of insect eyes are controlled by duplicated Pax-6 genes. Cephalopods belong to the Protostomes but possess a camera-type eye similar to those in vertebrates. We examined Pax-6 variations in the squid and found five types of Pax-6 splicing variants but no duplication of the Pax-6 gene. In the five splicing variants, the splicing patterns were produced by the combination of two additional exons to the ortholog and one jettisoned exon containing most of the Homeobox domain (HD). These five variants show spatio-temporal patterns of gene expression during development in the squid. Our study suggests that cephalopods acquired Pax-6 splicing variants independent of those in vertebrates and that these variants were similarly utilized in the development of the squid eye.

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0 (Eye Proteins)
0 (Homeodomain Proteins)
0 (PAX6 Transcription Factor)
0 (Paired Box Transcription Factors)
0 (Repressor Proteins)

Date Created: 20140306 Date Completed: 20151009 Latest Revision: 20211021

20231215

PMC3942700

10.1038/srep04256

24594543