Molecular and immunogenic properties of apyrase SP01B and D7-related SP04 recombinant salivary proteins of Phlebotomus perniciosus from Madrid, Spain.

Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE

Original Publication: New York, NY : Hindawi Pub. Co.

Apyrase/*genetics ; Leishmania infantum/*genetics ; Leishmaniasis/*genetics ; Phlebotomus/*genetics ; Salivary Proteins and Peptides/*genetics; Animals ; Apyrase/immunology ; Apyrase/isolation & purification ; Base Sequence ; Cricetinae ; Gene Library ; Leishmania infantum/pathogenicity ; Leishmaniasis/immunology ; Leishmaniasis/prevention & control ; Leishmaniasis/transmission ; Mice ; Phlebotomus/immunology ; Phlebotomus/parasitology ; Phylogeny ; Saliva/metabolism ; Saliva/parasitology ; Salivary Proteins and Peptides/immunology ; Salivary Proteins and Peptides/isolation & purification ; Sequence Analysis, DNA ; Spain

Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties, producing recombinant proteins of different vectors has become a major task. In this study, a cDNA library was constructed with the salivary glands of Phlebotomus perniciosus from Madrid, Spain, the most widespread vector of Leishmania infantum in the Mediterranean basin. Analysis of the cDNA sequences showed several polymorphisms among the previously described salivary transcripts. The apyrase SP01B and the D7-related protein SP04 were successfully cloned, expressed in Escherichia coli, and purified. Besides, recombinant proteins were recognized by sera of hamsters and mice previously immunized with saliva through the exposure to uninfected sand fly bites. These results suggest that these two recombinant proteins conserved their immunogenic properties after expression in a prokaryote system. Therefore, this work contributes to expand the knowledge of P. perniciosus saliva that would be eventually used for the development of tools for vector control programs.

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0 (Salivary Proteins and Peptides)
EC 3.6.1.5 (Apyrase)

Date Created: 20131031 Date Completed: 20140609 Latest Revision: 20211021

20240628

PMC3793307

10.1155/2013/526069

24171166