The impact of humic and fulvic acids on the dynamic properties of liposome membranes: the ESR method.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9001952 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2394 (Electronic) Linking ISSN: 08982104 NLM ISO Abbreviation: J Liposome Res Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: New York, N.Y. : Dekker, c1988-

Humic Substances* ; Lipid Bilayers*; Benzopyrans/*chemistry ; Liposomes/*chemistry; Electron Spin Resonance Spectroscopy/methods ; Free Radicals/analysis ; Kinetics ; Membrane Fluidity ; Nuclear Magnetic Resonance, Biomolecular ; Spin Labels

This paper presents the results of research on the influence of two fractions of humic substances (HS): fulvic acids (FA) and humic acids (HA), as a function of concentration, on the liposome membranes formed from egg yolk lecithin (EYL). The concentration of HS in relation to EYL changed from 0% to 10% by weight. The influence of HS on various areas of membranes: interphase water-lipid, in the lipid layer just below the polar part of the membrane and in the middle of the lipid bilayer, was investigated by different spin labels (TEMPO, DOXYL 5, DOXYL 16). The study showed that HA slightly decreased the fluidity of the analyzed membranes on the surface layer, while FA significantly liquidated the center of the lipid bilayer. The strong effect of both fractions of HS on the concentration of free radicals as a function of time was also described.

Keywords: ESR method; EYL liposomes; humic substances

0 (Benzopyrans)
0 (Free Radicals)
0 (Humic Substances)
0 (Lipid Bilayers)
0 (Liposomes)
0 (Spin Labels)
XII14C5FXV (fulvic acid)

Date Created: 20131023 Date Completed: 20150406 Latest Revision: 20140702

20231215

10.3109/08982104.2013.839998

24144352