Acute and Subchronic Toxicity Study of Tamra Bhasma (Incinerated Copper) prepared from Ashodhita (Unpurified) and Shodhita (Purified) Tamra in Rats.

Publisher: OMICS International Country of Publication: India NLM ID: 7809431 Publication Model: Print Cited Medium: Print ISSN: 0250-474X (Print) Linking ISSN: 0250474X NLM ISO Abbreviation: Indian J Pharm Sci Subsets: PubMed not MEDLINE

Publication: Mar/April 2016- : Los Angeles, CA : OMICS International
Original Publication: Bombay : Indian Pharmaceutical Assn.

The use of metals in traditional medicines is very often seen as matter of concern these days, especially the Bhasma preparations which are always under stringent observations for containing highly reactive inorganic elements such as lead, mercury, arsenic and others. One of the Bhasma extensively used in routine Ayurvedic practice is Tamra (copper) bhasma. If it is not prepared properly or Shodhana procedure is not done properly, it acts as a poison. To indicate its toxic potential, Ashtamahadoshas (eight major ill effects) have been quoted in classics and due emphasis have been given to its Shodhana procedure. In the present study, Tamra bhasma prepared from Shodhita and Ashodhita Tamra was subjected to oral toxicity study to ascertain the role of Shodhana process on safety profile of Tamra bhasma on subchronic administration to albino rats. Both the samples were administered to rats for 45 consecutive days at the doses of 5.5, 27.5, and 55 mg/kg. Animals were sacrificed on 46(th) day and parameters like hematological, serum biochemical, and histopathology of various organs were studied. Results showed that Tamra bhasma prepared from Ashodhita Tamra has pathological implications on different hematological, serum biochemical and cytoarchitecture of different organs even at therapeutic dose level (5.5 mg/kg). Whereas, Tamra bhasma prepared from Shodhita Tamra is safe even at five-fold to therapeutic equivalent doses (27.5 mg/kg). These observations emphasize the role of Shodhana and importance of dose in expression of toxicity of the medicinal preparations.

Chemosphere. 2003 Sep;52(9):1361-71. (PMID: 12867165)
Br Med Bull. 2003;68:167-82. (PMID: 14757716)
Clin Chem. 1973 May;19(5):522-4. (PMID: 4703662)
Indian J Clin Biochem. 2003 Jul;18(2):181-9. (PMID: 23105410)
Scand J Clin Lab Invest. 1965;17(4):381-7. (PMID: 5838275)
Klin Wochenschr. 1965 Feb 1;43:174-5. (PMID: 14258517)
Clin Chem. 1982 Oct;28(10):2077-80. (PMID: 6812986)
Z Klin Chem Klin Biochem. 1974 May;12(5):226. (PMID: 4440114)
Clin Chem. 1969 Jun;15(6):487-95. (PMID: 5786804)
Anc Sci Life. 2006 Jan;25(3-4):57-63. (PMID: 22557208)
Clin Chem. 1974 Apr;20(4):447-53. (PMID: 4818198)
J Ethnopharmacol. 2012 Jun 26;142(1):98-104. (PMID: 22561344)
Clin Chim Acta. 1973 Oct 12;48(2):193-201. (PMID: 4758882)

Keywords: Amrutikarana; LD50; Marana; Shodhana; copper; herbomineral formulation

Date Created: 20131002 Date Completed: 20131001 Latest Revision: 20211021

20240829

PMC3783753

10.4103/0250-474X.117433

24082351