The MRI contrast agent gadoteridol enhances distribution of rAAV1 in the rat hippocampus.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9421525 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5462 (Electronic) Linking ISSN: 09697128 NLM ISO Abbreviation: Gene Ther Subsets: MEDLINE

Publication: London : Nature Publishing Group
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Macmillan Press Ltd., c1994-

Transduction, Genetic*; Dependovirus/*genetics ; Dependovirus/*physiology ; Heterocyclic Compounds/*pharmacology ; Hippocampus/*virology ; Organometallic Compounds/*pharmacology; Animals ; Gadolinium/pharmacology ; Genetic Vectors ; Green Fluorescent Proteins/genetics ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; Viral Tropism

Contrast agents are commonly used in combination with magnetic resonance imaging (MRI) to monitor the distribution of molecules in the brain. Recent experiments conducted in our laboratory have shown that co-infusion of recombinant Adeno-associated virus serotype 5 (rAAV5) and the MRI contrast agent gadoteridol (Gd) enhances vector transduction in the rat striatum. The goal of this study was to determine whether gadoteridol may also be used as a tool to enhance transduction efficiency of rAAV1 and rAAV5 within the rat hippocampus. We show that Gd/rAAV1-GFP but not Gd/rAAV5-GFP co-infusion results in significantly higher distribution of the transgene both in the injected hemisphere as well as in the contralateral side and adjacent areas of cortex along the injection track. We also show that Gd/rAAV1-GFP co-infusion has no deleterious effect on hippocampal function as assessed by two tests of spatial memory formation. This work indicates that Gd can be exploited as a method to increase transduction efficiency of AAV1 in the hippocampus for animal studies.

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T32 GM007507 United States GM NIGMS NIH HHS; T32GM007507 United States GM NIGMS NIH HHS

0 (Heterocyclic Compounds)
0 (Organometallic Compounds)
0 (Recombinant Proteins)
0199MV609F (gadoteridol)
147336-22-9 (Green Fluorescent Proteins)
AU0V1LM3JT (Gadolinium)

Date Created: 20130920 Date Completed: 20140713 Latest Revision: 20211021

20250114

PMC3855498

10.1038/gt.2013.47

24048419