A divergent Artiodactyl MYADM-like repeat is associated with erythrocyte traits and weight of lamb weaned in domestic sheep.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Weaning*; Body Weight/*genetics ; Myelin and Lymphocyte-Associated Proteolipid Proteins/*genetics ; Polymorphism, Single Nucleotide/*genetics ; Repetitive Sequences, Nucleic Acid/*genetics ; Sheep, Domestic/*genetics; Alleles ; Animals ; Chromosomes, Mammalian/genetics ; Erythrocyte Indices/genetics ; Erythrocytes ; Gene Frequency/genetics ; Genome/genetics ; Genome-Wide Association Study ; Genotype ; Hemoglobins/metabolism ; Phenotype ; Quantitative Trait, Heritable

A genome-wide association study (GWAS) was performed to investigate seven red blood cell (RBC) phenotypes in over 500 domestic sheep (Ovis aries) from three breeds (Columbia, Polypay, and Rambouillet). A single nucleotide polymorphism (SNP) showed genome-wide significant association with increased mean corpuscular hemoglobin concentration (MCHC, P = 6.2×10(-14)) and genome-wide suggestive association with decreased mean corpuscular volume (MCV, P = 2.5×10(-6)). The ovine HapMap project found the same genomic region and the same peak SNP has been under extreme historical selective pressure, demonstrating the importance of this region for survival, reproduction, and/or artificially selected traits. We observed a large (>50 kb) variant haplotype sequence containing a full-length divergent artiodactyl MYADM-like repeat in strong linkage disequilibrium with the associated SNP. MYADM gene family members play roles in membrane organization and formation in myeloid cells. However, to our knowledge, no member of the MYADM gene family has been identified in development of morphologically variant RBCs. The specific RBC differences may be indicative of alterations in morphology. Additionally, erythrocytes with altered morphological structure often exhibit increased structural fragility, leading to increased RBC turnover and energy expenditure. The divergent artiodactyl MYADM-like repeat was also associated with increased ewe lifetime kilograms of lamb weaned (P = 2×10(-4)). This suggests selection for normal RBCs might increase lamb weights, although further validation is required before implementation in marker-assisted selection. These results provide clues to explain the strong selection on the artiodactyl MYADM-like repeat locus in sheep, and suggest MYADM family members may be important for RBC morphology in other mammals.

Mol Biol Cell. 2011 Apr 15;22(8):1252-62. (PMID: 21325632)
Anim Genet. 2010 Apr;41(2):222-3. (PMID: 19839985)
Trends Biochem Sci. 2002 Dec;27(12):599-601. (PMID: 12468223)
Science. 2010 Jul 2;329(5987):72-5. (PMID: 20466884)
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9362-7. (PMID: 19474294)
Blood. 1984 Jun;63(6):1353-60. (PMID: 6722353)
Genet Res (Camb). 2010 Dec;92(5-6):413-21. (PMID: 21429272)
PLoS One. 2012;7(10):e47829. (PMID: 23082221)
Nat Genet. 2009 Nov;41(11):1191-8. (PMID: 19862010)
Br J Haematol. 1999 Jan;104(1):2-13. (PMID: 10027705)
Anim Genet. 2009 Oct;40(5):583-9. (PMID: 19397512)
Cell. 2004 Aug 20;118(4):477-91. (PMID: 15315760)
Anim Genet. 2013 Feb;44(1):34-43. (PMID: 22548415)
Immunogenetics. 2008 Dec;60(12):749-58. (PMID: 18797863)
Bioinformatics. 2010 Mar 1;26(5):589-95. (PMID: 20080505)
Lancet. 1986 Nov 22;2(8517):1198-201. (PMID: 2430154)
Blood Cells Mol Dis. 1997 Dec;23(3):417-21. (PMID: 9446757)
Hematology Am Soc Hematol Educ Program. 2005;:13-8. (PMID: 16304353)
Nat Genet. 2009 Nov;41(11):1182-90. (PMID: 19820697)
Am J Hematol. 2007 Jul;82(7):605-10. (PMID: 17211848)
Anim Genet. 2010 Oct;41(5):449-53. (PMID: 20809919)
Mol Biol Rep. 2005 Sep;32(3):149-57. (PMID: 16172915)
Genome. 2010 Nov;53(11):876-83. (PMID: 21076503)
Am J Phys Anthropol. 2000 Feb;111(2):165-76. (PMID: 10640944)
Cell Mol Life Sci. 2008 Jan;65(1):92-9. (PMID: 17917702)
J Anim Sci. 2011 Aug;89(8):2297-309. (PMID: 21421834)
Genome Biol. 2009;10(4):R42. (PMID: 19393038)
Genome Res. 2012 Apr;22(4):778-90. (PMID: 22300768)
Nat Biotechnol. 2013 Feb;31(2):135-41. (PMID: 23263233)
PLoS Biol. 2012 Feb;10(2):e1001258. (PMID: 22346734)
Mol Biol Rep. 2001;28(3):123-38. (PMID: 12075932)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Anim Genet. 2012 Feb;43(1):63-71. (PMID: 22221026)
J Clin Invest. 1970 Apr;49(4):623-34. (PMID: 5443167)
Twin Res. 1999 Dec;2(4):250-7. (PMID: 10723803)
Am J Hum Genet. 2007 Sep;81(3):559-75. (PMID: 17701901)
Nat Rev Genet. 2008 May;9(5):356-69. (PMID: 18398418)
Blood Cells Mol Dis. 1998 Dec;24(4):539-43. (PMID: 9887280)
J Leukoc Biol. 2000 Mar;67(3):423-31. (PMID: 10733104)
Genet Epidemiol. 1985;2(2):133-44. (PMID: 4054596)
Science. 1997 Oct 31;278(5339):876-8. (PMID: 9346488)

0 (Hemoglobins)
0 (Myelin and Lymphocyte-Associated Proteolipid Proteins)

Date Created: 20130912 Date Completed: 20140415 Latest Revision: 20211021

20240829

PMC3758307

10.1371/journal.pone.0074700

24023702