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Kallikrein gene-modified EPCs induce angiogenesis in rats with ischemic hindlimb and correlate with integrin αvβ3 expression.

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  • المؤلفون: Fu SS;Fu SS; Li FJ; Wang YY; You AB; Qie YL; Meng X; Li JR; Li BC; Zhang Y; Da Li Q
  • المصدر:
    PloS one [PLoS One] 2013 Sep 03; Vol. 8 (9), pp. e73035. Date of Electronic Publication: 2013 Sep 03 (Print Publication: 2013).
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Neovascularization, Pathologic*; Endothelium/*pathology ; Hindlimb/*blood supply ; Integrin alphaVbeta3/*genetics ; Ischemia/*genetics ; Kallikreins/*genetics ; Stem Cells/*pathology; Animals ; Female ; Integrin alphaVbeta3/metabolism ; Ischemia/metabolism ; Nitric Oxide Synthase Type III/genetics ; Rats ; Rats, Wistar ; Transfection
    • نبذة مختصرة :
      Background: Human tissue kallikrein (hTK) plays an essential role in the physiological and pathological mechanisms of blood vessels. This study aimed to determine whether angiogenesis induced by endothelial progenitor cells (EPCs) transduced with the adenovirus-mediated hTK gene could improve blood flow in rat hindlimb ischemia in vivo and to establish a promising mechanism in vitro.
      Methods: EPCs transduced with adenovirus encoding hTK-162 (i.e., Ad/hTK-transduced EPCs or Ad/GFP-transduced EPCs) were administered to Wister rats with hindlimb ischemia through therapeutic neovascularization. Muscular capillary density (MCD), blood flow (BF), and the number of myofibers were measured at days 7, 14, and 21 after treatment. Expressions of integrin αvβ3 and endothelial nitric oxide synthase (eNOS) were detected on the surface of EPCs.
      Results: MCD, BF, and the number of myofibers in rats with Ad/hTK-transduced EPCs remarkably increased at day 21 after treatment compared with rats with Ad/GFP-transduced EPCs or the control group (P<0.01). Expressions of integrin αvβ3 and eNOS protein on the surface of EPCs also increased in rats with Ad/hTK-transduced EPCs. The levels of integrin αvβ3 expression were reduced by PI3K and eNOS blockade, and the inhibitor of integrin αvβ3 abrogated the migration and adhesion of hTK-transduced EPCs (P<0.05).
      Conclusion: hTK gene delivery in vivo improves the natural angiogenic response to ischemia. The ability of hTK gene-transduced EPCs can be enhanced in vitro, in which integrin αvβ3 plays a role in the process.
    • References:
      Cardiovasc Res. 2009 Jan 1;81(1):159-68. (PMID: 18806276)
      J Biol Chem. 2008 Oct 31;283(44):30363-75. (PMID: 18765664)
      Trends Cardiovasc Med. 2005 Feb;15(2):57-63. (PMID: 15885571)
      Circulation. 2002 Feb 12;105(6):732-8. (PMID: 11839630)
      Circ Res. 2011 Feb 4;108(3):284-93. (PMID: 21164105)
      Ann Neurol. 2010 Apr;67(4):488-97. (PMID: 20437584)
      Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3422-7. (PMID: 10725398)
      Blood. 2004 Oct 1;104(7):2010-9. (PMID: 15213103)
      Br J Pharmacol. 1998 Jul;124(6):1286-92. (PMID: 9720802)
      Arterioscler Thromb Vasc Biol. 1999 May;19(5):1156-61. (PMID: 10323764)
      Nat Rev Drug Discov. 2007 Apr;6(4):273-86. (PMID: 17396134)
      Stroke. 1999 Sep;30(9):1925-31; discussion 1931-2. (PMID: 10471446)
      Am J Pathol. 2010 Jan;176(1):504-15. (PMID: 19948824)
      Microvasc Res. 2010 May;79(3):200-6. (PMID: 20144623)
      Hypertension. 2000 Jan;35(1 Pt 1):25-31. (PMID: 10642270)
      Fibrogenesis Tissue Repair. 2012 Mar 12;5:4. (PMID: 22410175)
      Nat Rev Mol Cell Biol. 2007 Nov;8(11):857-69. (PMID: 17895899)
      Atherosclerosis. 2007 Apr;191(2):259-64. (PMID: 16787646)
      Arterioscler Thromb Vasc Biol. 2000 Nov;20(11):2379-85. (PMID: 11073841)
      Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11951-6. (PMID: 12195016)
      Arterioscler Thromb Vasc Biol. 2009 May;29(5):657-64. (PMID: 19164804)
      Hypertension. 2004 Aug;44(2):203-9. (PMID: 15238569)
      Microsc Res Tech. 2000 Nov 1;51(3):280-301. (PMID: 11054877)
      Science. 2008 Jan 11;319(5860):195-8. (PMID: 18187653)
      Circulation. 2004 May 25;109(20):2454-61. (PMID: 15148275)
      Acta Med Croatica. 2012 Oct;66 Suppl 1:19-24. (PMID: 23193816)
      Curr Top Dev Biol. 2004;64:207-38. (PMID: 15563949)
      Diabetes Care. 2009 Jan;32(1):117-9. (PMID: 18835949)
      Nat Med. 2001 Apr;7(4):430-6. (PMID: 11283669)
      Circulation. 2004 Jun 8;109(22):2692-7. (PMID: 15184293)
      Circ Res. 2008 Aug 29;103(5):536-44. (PMID: 18658052)
      Nature. 2005 Dec 15;438(7070):937-45. (PMID: 16355211)
      Dev Biol. 2005 Nov 15;287(2):390-402. (PMID: 16216232)
      Int Wound J. 2013 Oct;10(5):527-33. (PMID: 22738265)
      J Vasc Surg. 2004 Mar;39(3):655-60. (PMID: 14981463)
      J Exp Med. 2005 Jun 6;201(11):1825-35. (PMID: 15928198)
      J Thromb Haemost. 2010 Jan;8(1):185-93. (PMID: 19874467)
      Gastroenterology. 2007 Jul;133(1):91-107.e1. (PMID: 17631135)
      Med Clin (Barc). 2008 Nov 15;131(17):665-9. (PMID: 19087795)
      Angiogenesis. 2012 Sep;15(3):511-9. (PMID: 22581517)
    • الرقم المعرف:
      0 (Integrin alphaVbeta3)
      EC 1.14.13.39 (Nitric Oxide Synthase Type III)
      EC 3.4.21.- (Kallikreins)
    • الموضوع:
      Date Created: 20130911 Date Completed: 20140516 Latest Revision: 20211021
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC3760867
    • الرقم المعرف:
      10.1371/journal.pone.0073035
    • الرقم المعرف:
      24019890