Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis.

Publisher: Wiley Country of Publication: England NLM ID: 101304007 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2659 (Electronic) Linking ISSN: 17502640 NLM ISO Abbreviation: Influenza Other Respir Viruses Subsets: MEDLINE

Original Publication: Oxford, UK : Wiley, c2007-

Influenza A virus/*growth & development ; Influenza in Birds/*transmission ; Orthomyxoviridae Infections/*transmission ; Polysaccharides/*analysis ; Respiratory System/*chemistry; Animals ; Birds ; China ; Glycomics ; Histocytochemistry ; Influenza A virus/isolation & purification ; Influenza in Birds/virology ; Organ Culture Techniques ; Orthomyxoviridae Infections/virology ; Respiratory System/virology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Swine

Objectives: Swine have been regarded as intermediate hosts in the spread of influenza from birds to humans but studies of the sialylated glycans that comprise their respiratory tract have not been extensively studied in the past. This study analyzed the sialylated N-glycan and O-glycan profile of swine trachea and lung and correlated this with ex-vivo infection of swine explants with avian influenza viruses.
Sample: Lungs and tracheal samples were obtained from normal farm and laboratory raised swine and used for ex vivo infection as well as mass spectrometric analysis. Infection of the ex vivo tissues used high pathogenic and low pathogenic avian viruses including the novel H7N9 virus that emerged in China in early 2013.
Main Outcome Measures: Assessment of successful replication was determined by TCID50 as well as virus immunohistochemistry. The N-glycan and O-glycan profiles were measured by MALDI-TOF and sialylated linkages were determined by sialidase treatment. Lectin binding histochemistry was also performed on formalin fixed tissue samples with positive binding detected by chromogen staining.
Results: The swine respiratory tract glycans differed from the human respiratory tact glycans in two main areas. There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans. The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract. Infection with avian viruses was confined primarily to lung bronchioles rather than trachea and parenchyma.
Conclusions: In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea. Infection of Siaα2-3 binding avian viruses was restricted to the lower respiratory tract bronchioles. This finding may diminish the ability of the swine to act as an intermediary in the transmission of avian viruses to humans.
(© 2013 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.)

Virology. 1985 Dec;147(2):287-94. (PMID: 2416114)
Virology. 2012 Jan 5;422(1):105-13. (PMID: 22056389)
Acta Histochem Suppl. 1990;40:35-8. (PMID: 2091044)
Virology. 2011 Apr 10;412(2):401-10. (PMID: 21333316)
Am J Respir Cell Mol Biol. 2004 Jun;30(6):871-9. (PMID: 14672916)
Glycobiology. 2011 Aug;21(8):988-93. (PMID: 21863598)
Respir Res. 2007 Oct 25;8:73. (PMID: 17961210)
Nature. 2011 May 26;473(7348):519-22. (PMID: 21614079)
Virology. 1983 Jun;127(2):361-73. (PMID: 6868370)
Glycobiology. 2007 Apr;17(4):411-22. (PMID: 17223647)
J Virol. 1998 Sep;72(9):7367-73. (PMID: 9696833)
J Biol Chem. 1990 Apr 25;265(12):7055-61. (PMID: 2108966)
J Gen Virol. 1994 Sep;75 ( Pt 9):2183-8. (PMID: 8077918)
Nature. 2009 Aug 20;460(7258):1021-5. (PMID: 19672242)
Microbiol Immunol. 2005;49(12):1063-7. (PMID: 16365531)
Angew Chem Int Ed Engl. 2012 May 14;51(20):4860-3. (PMID: 22505324)
Virol J. 2010 Feb 16;7:38. (PMID: 20158900)
Nature. 1983 Jul 7-13;304(5921):76-8. (PMID: 6191220)
Science. 2013 Jul 12;341(6142):183-6. (PMID: 23704376)
Antimicrob Agents Chemother. 2009 Sep;53(9):3935-41. (PMID: 19596886)
Emerg Infect Dis. 2010 Oct;16(10):1515-23. (PMID: 20875275)
J Biol Chem. 2010 Oct 29;285(44):34016-26. (PMID: 20724471)
Nature. 2006 Mar 23;440(7083):435-6. (PMID: 16554799)
Virology. 2011 Apr 25;413(1):139-47. (PMID: 21397290)
Influenza Other Respir Viruses. 2013 Mar;7(2):151-9. (PMID: 22564359)
Vaccine. 2009 Sep 18;27(41):5620-7. (PMID: 19647064)
Am J Pathol. 2010 Apr;176(4):1828-40. (PMID: 20110407)
Nature. 2012 May 02;486(7403):420-8. (PMID: 22722205)
Anal Biochem. 2007 Dec 1;371(1):52-61. (PMID: 17632070)
J Magn Reson Imaging. 2009 Feb;29(2):305-12. (PMID: 19161181)
Virus Res. 2008 May;133(2):269-79. (PMID: 18329747)
Science. 2012 Jun 22;336(6088):1534-41. (PMID: 22723413)
Virol J. 2007 May 09;4:42. (PMID: 17490484)
Methods Enzymol. 2006;415:59-86. (PMID: 17116468)
Nat Med. 2007 Feb;13(2):147-9. (PMID: 17206149)
PLoS Pathog. 2008 Jul 11;4(7):e1000102. (PMID: 18617994)
Science. 2012 Jun 22;336(6088):1541-7. (PMID: 22723414)
BMC Vet Res. 2010 Jan 27;6:4. (PMID: 20105300)
Vet Res. 2007 Mar-Apr;38(2):243-60. (PMID: 17257572)
PLoS One. 2009 Dec 23;4(12):e8431. (PMID: 20037716)
Influenza Other Respir Viruses. 2013 Nov;7(6):1269-82. (PMID: 24001121)
Am J Pathol. 2007 Oct;171(4):1215-23. (PMID: 17717141)
Virol J. 2011 Sep 08;8:434. (PMID: 21902821)

United Kingdom Wellcome Trust; 082098 United Kingdom WT_ Wellcome Trust

Keywords: Glycomics; influenza A virus; swine

0 (Polysaccharides)

Date Created: 20130905 Date Completed: 20140618 Latest Revision: 20211021

20231215

PMC4114536

10.1111/irv.12144

24001121