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Physiology and gene expression profiles of Dekkera bruxellensis in response to carbon and nitrogen availability.
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- معلومة اضافية
- المصدر:
Publisher: Springer Country of Publication: Netherlands NLM ID: 0372625 Publication Model: Print Cited Medium: Internet ISSN: 1572-9699 (Electronic) Linking ISSN: 00036072 NLM ISO Abbreviation: Antonie Van Leeuwenhoek Subsets: MEDLINE
- بيانات النشر:
Publication: 2004- : Berlin : Springer
Original Publication: Wageningen, Netherland [etc.] Veenman [etc.]
- الموضوع:
- نبذة مختصرة :
The assimilation of nitrate, a nitrogenous compound, was previously described as an important factor favoring Dekkera bruxellensis in the competition with Saccharomyces cerevisiae for the industrial sugarcane substrate. In this substrate, nitrogen sources are limited and diverse, and a recent report showed that amino acids enable D. bruxellensis to grow anaerobically. Thus, understanding the regulation of nitrogen metabolism is one fundamental aspect to comprehend the competiveness of D. bruxellensis in the fermentation environment. In the present study, we evaluated the physiological and transcriptional profiles of D. bruxellensis in response to different carbon and nitrogen supplies to determine their influence on growth, sugar consumption, and ethanol production. Besides, the expression of genes coding for nitrogen permeases and enzymes involved in the biosynthesis of glutamate and energetic metabolism were investigated under these conditions. Our data revealed that genes related to nitrogen uptake in D. bruxellensis are under the control of nitrogen catabolite repression. Moreover, we provide indications that glutamate dehydrogenase and glutamate synthase may switch roles as the major pathway for glutamate biosynthesis in D. bruxellensis. Finally, our data showed that in nonoptimal growth conditions, D. bruxellensis leans toward the respiratory metabolism. The results presented herein show that D. bruxellensis and S. cerevisiae share similar regulation of GDH–GOGAT pathway, while D. bruxellensis converts less glucose to ethanol than S. cerevisiae do when nitrogen is limited. The consequence of this particularity to the industrial process is discussed.
- الرقم المعرف:
3K9958V90M (Ethanol)
7440-44-0 (Carbon)
N762921K75 (Nitrogen)
- الموضوع:
Date Created: 20130821 Date Completed: 20140526 Latest Revision: 20131127
- الموضوع:
20240628
- الرقم المعرف:
10.1007/s10482-013-9998-x
- الرقم المعرف:
23959165
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