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Genome-wide profiling of human cap-independent translation-enhancing elements.

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  • معلومة اضافية
    • المصدر:
      Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: New York, NY : Nature Pub. Group, c2004-
    • الموضوع:
      Genome, Human* ; Peptide Chain Initiation, Translational*; RNA, Messenger/*genetics; 5' Untranslated Regions ; Gene Library ; HeLa Cells ; High-Throughput Nucleotide Sequencing ; Humans ; Protein Biosynthesis ; RNA, Messenger/metabolism
    • نبذة مختصرة :
      We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
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    • Grant Information:
      GM085530 United States GM NIGMS NIH HHS; R01 HG002096 United States HG NHGRI NIH HHS; R01 GM085530 United States GM NIGMS NIH HHS; R01 AI095394 United States AI NIAID NIH HHS; HG002096-11 United States HG NHGRI NIH HHS
    • الرقم المعرف:
      0 (5' Untranslated Regions)
      0 (RNA, Messenger)
    • الموضوع:
      Date Created: 20130618 Date Completed: 20131211 Latest Revision: 20211021
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC3731418
    • الرقم المعرف:
      10.1038/nmeth.2522
    • الرقم المعرف:
      23770754