Integrated metagenomic and physiochemical analyses to evaluate the potential role of microbes in the sand filter of a drinking water treatment system.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Silicon Dioxide*; Biofilms/*growth & development ; Filtration/*instrumentation ; Groundwater/*microbiology ; Metagenome/*genetics ; Proteobacteria/*genetics ; Water Purification/*instrumentation; Base Sequence ; China ; Cluster Analysis ; Computational Biology ; Drinking Water ; Groundwater/chemistry ; Hydrogen-Ion Concentration ; Metals, Heavy/analysis ; Molecular Sequence Annotation ; Molecular Sequence Data ; Photoelectron Spectroscopy ; Phylogeny ; Proteobacteria/classification ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA

While sand filters are widely used to treat drinking water, the role of sand filter associated microorganisms in water purification has not been extensively studied. In the current investigation, we integrated molecular (based on metagenomic) and physicochemical analyses to elucidate microbial community composition and function in a common sand filter used to treat groundwater for potable consumption. The results revealed that the biofilm developed rapidly within 2 days (reaching ≈ 10(11) prokaryotes per gram) in the sand filter along with abiotic and biotic particulates accumulated in the interstitial spaces. Bacteria (up to 90%) dominated the biofilm microbial community, with Alphaproteobacteria being the most common class. Thaumarchaeota was the sole phylum of Archaea, which might be involved in ammonia oxidation. Function annotation of metagenomic datasets revealed a number of aromatic degradation pathway genes, such as aromatic oxygenase and dehydrogenase genes, in the biofilm, suggesting a significant role for microbes in the breakdown of aromatic compounds in groundwater. Simultaneous nitrification and denitrification pathways were confirmed as the primary routes of nitrogen removal. Dissolved heavy metals in groundwater, e.g. Mn(2+) and As(3+), might be biologically oxidized to insoluble or easily adsorbed compounds and deposited in the sand filter. Our study demonstrated that the role of the microbial community in the sand filter treatment system are critical to effective water purification in drinking water.

FEMS Microbiol Ecol. 2012 May;80(2):323-30. (PMID: 22611552)
Bioinformatics. 2010 Mar 15;26(6):715-21. (PMID: 20130030)
ISME J. 2012 Jan;6(1):81-93. (PMID: 21716304)
Nature. 2010 Mar 4;464(7285):59-65. (PMID: 20203603)
Appl Environ Microbiol. 2006 Sep;72(9):5702-12. (PMID: 16957184)
Environ Microbiol. 2008 Oct;10(10):2728-45. (PMID: 18637950)
Arch Microbiol. 2011 Feb;193(2):89-93. (PMID: 21063867)
ISME J. 2011 May;5(5):918-28. (PMID: 21160538)
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14683-8. (PMID: 16186488)
Water Res. 2002 Nov;36(18):4543-51. (PMID: 12418657)
Microbes Environ. 2011;26(2):149-55. (PMID: 21502735)
Water Res. 2005 Jan-Feb;39(2-3):331-9. (PMID: 15644241)
Biotechnol Bioeng. 2006 Jun 5;94(2):209-22. (PMID: 16598794)
J Bacteriol. 2011 Oct;193(19):5539-40. (PMID: 21914867)
PLoS One. 2012;7(1):e30058. (PMID: 22253877)
Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17240-5. (PMID: 20855593)
Environ Microbiol. 2010 Sep;12(9):2613-23. (PMID: 20482588)
Appl Environ Microbiol. 2009 Dec;75(23):7537-41. (PMID: 19801464)
J Hazard Mater. 2003 Jan 31;96(2-3):291-303. (PMID: 12493214)
BMC Bioinformatics. 2008 Sep 19;9:386. (PMID: 18803844)
Chemosphere. 2011 Nov;85(7):1146-53. (PMID: 21974919)
Water Res. 2009 Apr;43(7):1801-9. (PMID: 19232671)
PLoS One. 2012;7(2):e31408. (PMID: 22363639)
BMC Microbiol. 2007 Nov 28;7:106. (PMID: 18045498)
Appl Microbiol Biotechnol. 2006 Oct;72(6):1322-9. (PMID: 16804694)
Appl Environ Microbiol. 2006 Mar;72(3):1858-72. (PMID: 16517632)
Curr Opin Microbiol. 2011 Jun;14(3):300-6. (PMID: 21546306)
Mol Biol Evol. 2011 Oct;28(10):2731-9. (PMID: 21546353)
Genome Res. 2008 May;18(5):821-9. (PMID: 18349386)
Environ Microbiol. 2007 Apr;9(4):934-43. (PMID: 17359265)
PLoS One. 2012;7(5):e37796. (PMID: 22666393)
AMB Express. 2012 Mar 27;2(1):18. (PMID: 22452812)
Microb Ecol. 2008 Jul;56(1):121-32. (PMID: 18034358)
Microb Inform Exp. 2012 Feb 09;2(1):3. (PMID: 22587947)
Appl Environ Microbiol. 2010 Feb;76(4):1224-31. (PMID: 20038702)
Water Res. 2010 Sep;44(17):5039-49. (PMID: 20673944)
Microb Ecol. 1992 Jul;24(1):91-108. (PMID: 24193042)

0 (Drinking Water)
0 (Metals, Heavy)
7631-86-9 (Silicon Dioxide)

Date Created: 20130418 Date Completed: 20131017 Latest Revision: 20211021

20240829

PMC3623876

10.1371/journal.pone.0061011

23593378