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Excisional wound healing is delayed in a murine model of chronic kidney disease.

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  • المؤلفون: Seth AK;Seth AK; De la Garza M; Fang RC; Hong SJ; Galiano RD
  • المصدر:
    PloS one [PLoS One] 2013; Vol. 8 (3), pp. e59979. Date of Electronic Publication: 2013 Mar 25.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: San Francisco, CA : Public Library of Science
    • الموضوع:
      Wound Healing*; Renal Insufficiency, Chronic/*pathology; Animals ; Cell Proliferation ; Disease Models, Animal ; Granulation Tissue/pathology ; Inflammation/genetics ; Inflammation/metabolism ; Inflammation/pathology ; Kidney Glomerulus/pathology ; Male ; Mice ; Neovascularization, Physiologic ; Renal Insufficiency, Chronic/genetics ; Renal Insufficiency, Chronic/metabolism ; Time Factors
    • نبذة مختصرة :
      Background: Approximately 15% of the United States population suffers from chronic kidney disease (CKD), often demonstrating an associated impairment in wound healing. This study outlines the development of a surgical murine model of CKD in order to investigate the mechanisms underlying this impairment.
      Methods: CKD was induced in mice by partial cauterization of one kidney cortex and contralateral nephrectomy, modifying a previously published technique. After a minimum of 6-weeks, splinted, dorsal excisional wounds were created to permit assessment of wound healing parameters. Wounds were harvested on postoperative days (POD) 0, 3, 7, and 14 for histological, immunofluorescent, and quantitative PCR (qPCR).
      Results: CKD mice exhibited deranged blood chemistry and hematology profiles, including profound uremia and anemia. Significant decreases in re-epithelialization and granulation tissue deposition rates were found in uremic mice wounds relative to controls. On immunofluorescent analysis, uremic mice demonstrated significant reductions in cellular proliferation (BrdU) and angiogenesis (CD31), with a concurrent increase in inflammation (CD45) as compared to controls. CKD mice also displayed differential expression of wound healing-related genes (VEGF, IL-1β, eNOS, iNOS) on qPCR.
      Conclusions: These findings represent the first reported investigation of cutaneous healing in a CKD animal model. Ongoing studies of this significantly delayed wound healing phenotype include the establishment of renal failure model in diabetic strains to study the combined effects of CKD and diabetes.
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    • الموضوع:
      Date Created: 20130329 Date Completed: 20131227 Latest Revision: 20211021
    • الموضوع:
      20231215
    • الرقم المعرف:
      PMC3607571
    • الرقم المعرف:
      10.1371/journal.pone.0059979
    • الرقم المعرف:
      23536900