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Stromal fibroblasts in the microenvironment of gastric carcinomas promote tumor metastasis via upregulating TAGLN expression.

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  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966972 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2121 (Electronic) Linking ISSN: 14712121 NLM ISO Abbreviation: BMC Cell Biol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, [2000-
    • الموضوع:
      Fibroblasts/*metabolism ; Microfilament Proteins/*metabolism ; Muscle Proteins/*metabolism ; Stomach Neoplasms/*metabolism; Aged ; Animals ; Cell Line ; Cell Movement ; Female ; Fibroblasts/cytology ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Matrix Metalloproteinase 2/genetics ; Matrix Metalloproteinase 2/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Microfilament Proteins/antagonists & inhibitors ; Microfilament Proteins/genetics ; Middle Aged ; Muscle Proteins/antagonists & inhibitors ; Muscle Proteins/genetics ; RNA Interference ; RNA, Small Interfering/metabolism ; Stomach Neoplasms/pathology ; Transplantation, Heterologous ; Tumor Microenvironment ; Up-Regulation
    • نبذة مختصرة :
      Background: Fibroblasts play a critical role in tumorigenesis, tumor progression and metastasis. However, their detailed molecular characteristics and clinical significance are still elusive. TAGLN is an actin-binding protein that plays an important role in tumorigenesis.
      Results: We investigated the interaction between cancer cells and the tumor microenvironment to determine how the fibroblasts from human gastric carcinoma facilitate tumorigenesis through TAGLN. QRT-PCR and Western blot indicated that TAGLN expression was upregulated in gastric carcinoma-associated fibroblasts (CAFs) that promote gastric cancer cell migration and invasion. Using small interfering RNA (siRNA), we found that CAFs enhanced tumor metastasis through upregulated TAGLN in vitro and in vivo. The expression of matrix metalloproteinase-2 (MMP-2) was significantly lower after TAGLN knock-down by siRNA. TAGLN levels were elevated in human gastric cancer stroma than normal gastric stroma and associated with differentiation and lymph node metastasis of gastric cancer.
      Conclusion: CAFs may promote gastric cancer cell migration and invasion via upregulating TAGLN and TAGLN induced MMP-2 production.
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    • الرقم المعرف:
      0 (Microfilament Proteins)
      0 (Muscle Proteins)
      0 (RNA, Small Interfering)
      0 (transgelin)
      EC 3.4.24.24 (Matrix Metalloproteinase 2)
    • الموضوع:
      Date Created: 20130321 Date Completed: 20130625 Latest Revision: 20220317
    • الموضوع:
      20240829
    • الرقم المعرف:
      PMC3610155
    • الرقم المعرف:
      10.1186/1471-2121-14-17
    • الرقم المعرف:
      23510049