Expression of Rac1, HIF-1α, and VEGF in gastric carcinoma: correlation with angiogenesis and prognosis.

Publisher: Karger Country of Publication: Switzerland NLM ID: 7808556 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0240 (Electronic) Linking ISSN: 0378584X NLM ISO Abbreviation: Onkologie Subsets: MEDLINE

Original Publication: Basel, New York, Karger.

Hypoxia-Inducible Factor 1, alpha Subunit/*analysis ; Neovascularization, Pathologic/*metabolism ; Neovascularization, Pathologic/*mortality ; Stomach Neoplasms/*metabolism ; Stomach Neoplasms/*mortality ; Vascular Endothelial Growth Factor A/*analysis ; rac1 GTP-Binding Protein/*analysis; Adult ; Aged ; Biomarkers, Tumor/analysis ; China/epidemiology ; Comorbidity ; Female ; Humans ; Male ; Middle Aged ; Neovascularization, Pathologic/diagnosis ; Prevalence ; Prognosis ; Reproducibility of Results ; Risk Assessment ; Sensitivity and Specificity ; Statistics as Topic ; Stomach Neoplasms/diagnosis ; Survival Analysis ; Survival Rate

Background: The aim of this study was to investigate the relationship between the expression of hypoxia-inducible factor-1α (HIF-1α), Ras-related C3 botulinum toxin substrate 1 (Rac1), and vascular endothelial growth factor (VEGF), as well as their correlation with angiogenesis and prognosis in gastric carcinoma.
Material and Methods: The expression of Rac1, HIF-1α, VEGF, and CD34 (described in terms of microvessel density, MVD) was determined by immunohistochemical staining of tissues from 60 radically resected gastric cancer specimens.
Results: The proportion of specimens expressing Rac1, HIF-1α, and VEGF was 37/60 (61.7%), 35/60 (58.3%), and 40/60 (66.7%), respectively. The levels of Rac1, HIF-1α, and VEGF expression were significantly correlated with Lauren's classification, lymph node metastasis, and pathologic staging (p < 0.05). There were positive correlations between MVD and the expression of Rac1, HIF-1α, and VEGF. The mean survival time and 5-year survival rate in cases with positive Rac1, HIF-1α, and VEGF expression and MVD ≥ 26.3 were significantly shorter than those with negative Rac1, HIF-1α, and VEGF expression and MVD < 26.3.
Conclusion: Rac1, HIF-1α, and VEGF play an important role in tumor invasion and metastasis, especially in tumor angiogenesis. Thus, testing the expression of Rac1, HIF-1α, and VEGF may be a useful index for treatment and prognosis.
(Copyright © 2013 S. Karger AG, Basel.)

0 (Biomarkers, Tumor)
0 (HIF1A protein, human)
0 (Hypoxia-Inducible Factor 1, alpha Subunit)
0 (RAC1 protein, human)
0 (VEGFA protein, human)
0 (Vascular Endothelial Growth Factor A)
EC 3.6.5.2 (rac1 GTP-Binding Protein)

Date Created: 20130315 Date Completed: 20130912 Latest Revision: 20151119

20221213

10.1159/000348525

23485997