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Preparation of highly concentrated influenza vaccine for use in novel delivery approaches.
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- معلومة اضافية
- المصدر:
Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE
- بيانات النشر:
Publication: 2016- : New York, NY : Elsevier
Original Publication: Easton, Pa., American Pharmaceutical Assn.
- الموضوع:
Antigens, Viral/
*administration & dosage ;
Antigens, Viral/
*isolation & purification ;
Hemagglutinin Glycoproteins, Influenza Virus/
*administration & dosage ;
Hemagglutinin Glycoproteins, Influenza Virus/
*isolation & purification ;
Influenza Vaccines/
*administration & dosage ;
Influenza Vaccines/
*isolation & purification ;
Orthomyxoviridae Infections/
*prevention & control;
Animals ;
Antibody Formation ;
Antigens, Viral/
immunology ;
Chromatography, Gel ;
Drug Delivery Systems ;
Electrophoresis, Polyacrylamide Gel ;
Female ;
Filtration/
instrumentation ;
Hemagglutinin Glycoproteins, Influenza Virus/
immunology ;
Humans ;
Influenza A Virus, H1N1 Subtype/
immunology ;
Influenza A Virus, H3N2 Subtype/
immunology ;
Influenza Vaccines/
immunology ;
Influenza, Human/
immunology ;
Influenza, Human/
prevention & control ;
Mice ;
Mice, Inbred BALB C ;
Orthomyxoviridae Infections/
immunology - نبذة مختصرة :
Vaccine antigens are usually available only as dilute solutions, which are difficult to formulate into various novel delivery systems, which often require highly concentrated antigens. To address this problem, we have utilized tangential flow filtration (TFF), a simple and scalable process to prepare highly concentrated vaccine antigens. Here, we describe the optimization of TFF to concentrate hemagglutinin (HA) of egg-derived influenza antigens, from 2008 to 2009 seasonal vaccine, to concentrations up to 28 mg/mL. Concentrated antigen was evaluated by single radial immunodiffusion and reversed-phase high-performance liquid chromatographic analysis for the estimation of the HA content and a range of assays including size exclusion, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and bicinchoninic acid assay for protein characterization. In addition, the concentrated antigens retained their immunogenicity, confirmed by the induction of immune responses comparable to that of unprocessed antigen in a mouse model. The liquid concentrates were stable for up to 4 weeks, which could allow subsequent formulation into novel delivery technologies. Hence, we have used influenza HA to demonstrate that the fast, robust, and scalable approach of TFF can be used to concentrate antigens for use in novel delivery approaches. Moreover, the concentration process could be applicable for a variety of antigens and a wide range of novel vaccine delivery applications.
(Copyright © 2013 Wiley Periodicals, Inc.)
- الرقم المعرف:
0 (Antigens, Viral)
0 (Hemagglutinin Glycoproteins, Influenza Virus)
0 (Influenza Vaccines)
- الموضوع:
Date Created: 20130111 Date Completed: 20130801 Latest Revision: 20130212
- الموضوع:
20231215
- الرقم المعرف:
10.1002/jps.23444
- الرقم المعرف:
23303584
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