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Assessment of retinal and choroidal blood flow changes using laser Doppler flowmetry in rats.

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  • المؤلفون: Hétu S;Hétu S; Pouliot M; Cordahi G; Couture R; Vaucher E
  • المصدر:
    Current eye research [Curr Eye Res] 2013 Jan; Vol. 38 (1), pp. 158-67. Date of Electronic Publication: 2012 Sep 19.
  • نوع النشر :
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 8104312 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2202 (Electronic) Linking ISSN: 02713683 NLM ISO Abbreviation: Curr Eye Res Subsets: MEDLINE
    • بيانات النشر:
      Publication: London : Informa Healthcare
      Original Publication: London : IRL Press, [c1981-
    • الموضوع:
      Choroid/*blood supply ; Laser-Doppler Flowmetry/*methods ; Regional Blood Flow/*physiology ; Retinal Artery/*physiopathology ; Retinal Artery Occlusion/*physiopathology; Animals ; Disease Models, Animal ; Laser Coagulation ; Male ; Mice ; Mice, Inbred C57BL ; Rats ; Rats, Wistar ; Reproducibility of Results ; Retinal Artery/pathology ; Retinal Artery/surgery ; Retinal Artery Occlusion/diagnosis ; Retinal Artery Occlusion/surgery
    • نبذة مختصرة :
      Purpose: A new noninvasive laser Doppler flowmetry (LDF) probe (one emitting fiber surrounded by a ring of eight collecting fibers, 1-mm interaxis distance) was tested for its sensitivity to assess the retinal/choroidal blood flow variations in response to hypercapnia, hyperoxia, diverse vasoactive agents and following retinal arteries photocoagulation in the rat.
      Materials and Methods: After pupil dilation, a LDF probe was placed in contact to the cornea of anesthetized rats in the optic axis. Hypercapnia and hyperoxia were induced by inhalation of CO(2) (8% in medical air) and O(2) (100%) while pharmacological agents were injected intravitreously. The relative contribution of the choroidal circulation to the LDF signal was estimated after retinal artery occlusion by photocoagulation.
      Results: Blood flow was significantly increased by hypercapnia (18%), adenosine (14%) and sodium nitroprusside (16%) as compared to baseline values while it was decreased by hyperoxia (-8%) and endothelin-1 (-11%). Photocoagulation of retinal arteries significantly decreased blood flow level (-45%).
      Conclusions: Although choroidal circulation most likely contributes to the LDF signal in this setting, the results demonstrate that LDF represents a suitable in vivo noninvasive technique to monitor online relative reactivity of retinal perfusion to metabolic or pharmacological challenge. This technique could be used for repeatedly assessing blood flow reactivity in rodent models of ocular diseases.
    • الموضوع:
      Date Created: 20120921 Date Completed: 20130604 Latest Revision: 20220409
    • الموضوع:
      20231215
    • الرقم المعرف:
      10.3109/02713683.2012.723296
    • الرقم المعرف:
      22992205