Melatonin promotes seminal root elongation and root growth in transgenic rice after germination.

Publisher: Wiley Country of Publication: England NLM ID: 8504412 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-079X (Electronic) Linking ISSN: 07423098 NLM ISO Abbreviation: J Pineal Res Subsets: MEDLINE

Publication: : Oxford : Wiley
Original Publication: New York : Liss, c1984-

Arylalkylamine N-Acetyltransferase/*metabolism ; Melatonin/*metabolism ; Oryza/*enzymology ; Plant Roots/*enzymology ; Plants, Genetically Modified/*enzymology ; Seedlings/*enzymology; Animals ; Arylalkylamine N-Acetyltransferase/biosynthesis ; Arylalkylamine N-Acetyltransferase/genetics ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Plant ; Genotype ; Germination ; Melatonin/pharmacology ; Oryza/drug effects ; Oryza/genetics ; Oryza/growth & development ; Phenotype ; Plant Roots/drug effects ; Plant Roots/growth & development ; Plants, Genetically Modified/drug effects ; Plants, Genetically Modified/growth & development ; Seedlings/drug effects ; Seedlings/growth & development ; Sheep ; Time Factors

The effect of melatonin on root growth after germination was examined in transgenic rice seedlings expressing sheep serotonin N-acetyltransferase (NAT). Enhanced melatonin levels were found in T(3) homozygous seedlings because of the ectopic overexpression of sheep NAT, which is believed to be the rate-limiting enzyme in melatonin biosynthesis in animals. Compared with wild-type rice seeds, the transgenic rice seeds showed enhanced seminal root growth and an analogous number of adventitious roots 4 and 10 days after seeding on half-strength Murashige and Skoog medium. The enhanced initial seminal root growth in the transgenic seedlings matched their increased root biomass well. We also found that treatment with 0.5 and 1 μM melatonin promoted seminal root growth of the wild type under continuous light. These results indicate that melatonin plays an important role in regulating both seminal root length and root growth after germination in monocotyledonous rice plants. This is the first report on the effects of melatonin on root growth in gain-of-function mutant plants that produce high levels of melatonin.
(© 2012 John Wiley & Sons A/S.)

EC 2.3.1.87 (Arylalkylamine N-Acetyltransferase)
JL5DK93RCL (Melatonin)

Date Created: 20120530 Date Completed: 20130322 Latest Revision: 20220318

20221213

10.1111/j.1600-079X.2012.01008.x

22640001