Mouse ficolin B has an ability to form complexes with mannose-binding lectin-associated serine proteases and activate complement through the lectin pathway.

Publisher: Hindawi Pub. Corp Country of Publication: United States NLM ID: 101135740 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1110-7251 (Electronic) Linking ISSN: 11107243 NLM ISO Abbreviation: J Biomed Biotechnol Subsets: MEDLINE

Publication: 2006- : Cairo : Hindawi Pub. Corp.
Original Publication: Akron, Ohio : Hindawi Pub. Corp., [2001-

Complement Pathway, Mannose-Binding Lectin/*physiology ; Lectins/*metabolism; Acetylglucosamine/chemistry ; Acetylglucosamine/metabolism ; Animals ; Blotting, Western ; Bone Marrow/chemistry ; Complement C4/metabolism ; Humans ; Lectins/chemistry ; Lectins/isolation & purification ; Mannose-Binding Protein-Associated Serine Proteases/chemistry ; Mannose-Binding Protein-Associated Serine Proteases/metabolism ; Mice ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Ficolins

Ficolins are thought to be pathogen-associated-molecular-pattern-(PAMP-) recognition molecules that function to support innate immunity. Like mannose-binding lectins (MBLs), most mammalian ficolins form complexes with MBL-associated serine proteases (MASPs), leading to complement activation via the lectin pathway. However, the ability of murine ficolin B, a homologue of human M-ficolin, to perform this function is still controversial. The results of the present study show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B, pulled down using GlcNAc-agarose, contained very low, but detectable, amounts of MASP-2 and small MBL-associated protein (sMAP) and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. These biochemical features of ficolin B were confirmed using recombinant mouse ficolin B produced in CHO cells. Taken together, these results suggest that like other mammalian homologues, murine ficolin B has an ability to exert its function via the lectin pathway.

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0 (Complement C4)
0 (Lectins)
0 (Recombinant Proteins)
EC 3.4.21.- (MASP-2 protein, mouse)
EC 3.4.21.- (Mannose-Binding Protein-Associated Serine Proteases)
V956696549 (Acetylglucosamine)

Date Created: 20120424 Date Completed: 20121002 Latest Revision: 20231213

20231215

PMC3306798

10.1155/2012/105891

22523468