In vitro blood-brain barrier models: current and perspective technologies.

Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE

Publication: 2016- : New York, NY : Elsevier
Original Publication: Easton, Pa., American Pharmaceutical Assn.

Blood-Brain Barrier*/anatomy & histology ; Blood-Brain Barrier*/physiology; Animals ; Brain/blood supply ; Chromatography ; Endothelial Cells/physiology ; Humans ; Membranes, Artificial ; Models, Biological ; Neuroglia/physiology ; Shear Strength

Even in the 21st century, studies aimed at characterizing the pathological paradigms associated with the development and progression of central nervous system diseases are primarily performed in laboratory animals. However, limited translational significance, high cost, and labor to develop the appropriate model (e.g., transgenic or inbred strains) have favored parallel in vitro approaches. In vitro models are of particular interest for cerebrovascular studies of the blood-brain barrier (BBB), which plays a critical role in maintaining the brain homeostasis and neuronal functions. Because the BBB dynamically responds to many events associated with rheological and systemic impairments (e.g., hypoperfusion), including the exposure of potentially harmful xenobiotics, the development of more sophisticated artificial systems capable of replicating the vascular properties of the brain microcapillaries are becoming a major focus in basic, translational, and pharmaceutical research. In vitro BBB models are valuable and easy to use supporting tools that can precede and complement animal and human studies. In this article, we provide a detailed review and analysis of currently available in vitro BBB models ranging from static culture systems to the most advanced flow-based and three-dimensional coculture apparatus. We also discuss recent and perspective developments in this ever expanding research field.
(Copyright © 2011 Wiley Periodicals, Inc.)

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R01 DA029121 United States DA NIDA NIH HHS; R01 DA029121-01A1 United States DA NIDA NIH HHS; R01 DA029121-02 United States DA NIDA NIH HHS; R01-DA029121-01A1 United States DA NIDA NIH HHS

0 (Membranes, Artificial)

Date Created: 20120104 Date Completed: 20120629 Latest Revision: 20211021

20240829

PMC3288147

10.1002/jps.23022

22213383