Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk.

Publisher: Springer Country of Publication: United States NLM ID: 1277355 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7438 (Electronic) Linking ISSN: 00494747 NLM ISO Abbreviation: Trop Anim Health Prod Subsets: MEDLINE

Publication: 2005- : Heidelberg : Springer
Original Publication: Edinburgh, Livingstone.

Cell Adhesion Molecules/*genetics ; Mastitis, Bovine/*microbiology ; Staphylococcal Infections/*veterinary ; Staphylococcus aureus/*genetics; Animals ; Bacterial Adhesion/genetics ; Biofilms ; Cattle ; Cell Adhesion Molecules/immunology ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Female ; India ; Mastitis, Bovine/genetics ; Mastitis, Bovine/immunology ; Milk/microbiology ; Nucleic Acid Hybridization ; Polymerase Chain Reaction/veterinary ; Staphylococcal Infections/genetics ; Staphylococcal Infections/immunology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/immunology ; Staphylococcus aureus/pathogenicity

Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.

Food Microbiol. 2008 Apr;25(2):304-12. (PMID: 18206773)
J Immunol. 2011 Jun 1;186(11):6585-96. (PMID: 21525381)
Eur J Cell Biol. 2010 Jan;89(1):103-11. (PMID: 19913940)
Vet Microbiol. 2009 Feb 16;134(1-2):106-12. (PMID: 18954946)
Trop Anim Health Prod. 2009 Dec;41(8):1643-51. (PMID: 19421889)
Vet Microbiol. 2005 May 20;107(3-4):295-9. (PMID: 15863290)
Res Vet Sci. 2012 Aug;93(1):82-7. (PMID: 21944833)
J Vet Sci. 2004 Jun;5(2):103-9. (PMID: 15192336)
J Dairy Sci. 2011 Jul;94(7):3305-10. (PMID: 21700015)
Vet Microbiol. 2007 Nov 15;125(1-2):141-9. (PMID: 17600640)
Vet J. 2006 May;171(3):398-407. (PMID: 16624706)
J Basic Microbiol. 2008 Aug;48(4):308-14. (PMID: 18720490)
J Vet Sci. 2007 Jun;8(2):151-4. (PMID: 17519568)
Vet Microbiol. 2003 Mar 20;92(1-2):179-85. (PMID: 12488081)
Am J Vet Res. 2011 Oct;72(10):1361-8. (PMID: 21962279)
Int J Food Microbiol. 2006 May 25;109(1-2):88-96. (PMID: 16503066)
Appl Environ Microbiol. 2003 Sep;69(9):5648-55. (PMID: 12957956)
Infect Immun. 1999 Oct;67(10):5427-33. (PMID: 10496925)
J Vet Sci. 2011 Mar;12(1):95-7. (PMID: 21368569)
Vet Res Commun. 2010 Jan;34(1):81-9. (PMID: 19902374)
J Clin Microbiol. 2001 Jun;39(6):2151-6. (PMID: 11376050)
FEMS Microbiol Lett. 2011 Mar;316(1):44-50. (PMID: 21204926)
J Bacteriol. 2001 May;183(9):2888-96. (PMID: 11292810)
Science. 1999 May 21;284(5418):1318-22. (PMID: 10334980)
Vet Microbiol. 2006 Nov 26;118(1-2):133-40. (PMID: 16920280)

0 (Cell Adhesion Molecules)
0 (DNA, Bacterial)

Date Created: 20111119 Date Completed: 20120508 Latest Revision: 20211021

20250114

10.1007/s11250-011-0009-9

22095031