Overexpression of the pepper antimicrobial protein CaAMP1 gene regulates the oxidative stress- and disease-related proteome in Arabidopsis.

Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE

Original Publication: Berlin, New York, Springer-Verlag [etc.]

Proteome*; Arabidopsis/*physiology ; Capsicum/*genetics ; Gene Expression Regulation, Plant/*physiology ; Plant Diseases/*immunology ; Plant Proteins/*genetics; Antioxidants/metabolism ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Arabidopsis/immunology ; Base Sequence ; Disease Resistance ; Down-Regulation ; Genes, Plant/genetics ; Hydrogen Peroxide/metabolism ; Molecular Sequence Data ; Oxidative Stress/genetics ; Paraquat/pharmacology ; Plant Diseases/microbiology ; Plant Leaves/physiology ; Plant Proteins/metabolism ; Plants, Genetically Modified ; Pseudomonas syringae/physiology ; Reactive Oxygen Species/metabolism ; Sequence Analysis, DNA ; Up-Regulation

Proteomics facilitates our understanding of cellular processes and network functions in the plant defense response during abiotic and biotic stresses. Here, we demonstrate that the ectopic expression of the Capsicum annuum antimicrobial protein CaAMP1 gene in Arabidopsis thaliana confers enhanced tolerance to methyl viologen (MV)-induced oxidative stress, which is accompanied by lower levels of lipid peroxidation. Quantitative comparative proteome analyses using two-dimensional gel electrophoresis coupled with mass spectrometry identified some of the oxidative stress- and disease-related proteins that are differentially regulated by CaAMP1 overexpression in Arabidopsis leaves. Antioxidant- and defense-related proteins, such as 2-cys peroxiredoxin, L-ascorbate peroxidase, peroxiredoxin, glutathione S-transferase and copper homeostasis factor, were up-regulated in the CaAMP1 transgenic leaf tissues. In contrast, GSH-dependent dehydroascorbate reductase and WD-40 repeat family protein were down-regulated by CaAMP1 overexpression. In addition, CaAMP1 overexpression enhanced resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 infection and also H(2)O(2) accumulation in Arabidopsis. The identified antioxidant- and defense-related genes were differentially expressed during MV-induced oxidative stress and Pst DC3000 infection. Taken together, we conclude that CaAMP1 overexpression can regulate the differential expression of defense-related proteins in response to environmental stresses to maintain reactive oxygen species (ROS) homeostasis.

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GENBANK AY548741

0 (Antioxidants)
0 (Plant Proteins)
0 (Proteome)
0 (Reactive Oxygen Species)
BBX060AN9V (Hydrogen Peroxide)
PLG39H7695 (Paraquat)

Date Created: 20110708 Date Completed: 20130503 Latest Revision: 20211020

20221213

10.1007/s00425-011-1473-1

21735195