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The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro.
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- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 100968565 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2474 (Electronic) Linking ISSN: 14712474 NLM ISO Abbreviation: BMC Musculoskelet Disord Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : BioMed Central, [2000-
- الموضوع:
- نبذة مختصرة :
Background: Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes in vitro.
Methods: Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions in vitro were investigated by transfection of a specific siRNA.
Results: Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli in vitro.
Conclusions: The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. In vitro experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.
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- الرقم المعرف:
0 (BIRC5 protein, human)
0 (Inhibitor of Apoptosis Proteins)
0 (RNA, Messenger)
0 (Survivin)
- الموضوع:
Date Created: 20110707 Date Completed: 20120419 Latest Revision: 20211020
- الموضوع:
20240628
- الرقم المعرف:
PMC3141611
- الرقم المعرف:
10.1186/1471-2474-12-150
- الرقم المعرف:
21729321
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