The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro.

Publisher: BioMed Central Country of Publication: England NLM ID: 100968565 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2474 (Electronic) Linking ISSN: 14712474 NLM ISO Abbreviation: BMC Musculoskelet Disord Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Cell Proliferation*; Cartilage, Articular/*metabolism ; Chondrocytes/*metabolism ; Chondrocytes/*pathology ; Chondrogenesis/*genetics ; Gene Expression Regulation/*genetics ; Inhibitor of Apoptosis Proteins/*genetics ; Osteoarthritis/*genetics; Aged ; Cartilage, Articular/pathology ; Cells, Cultured ; Chondrocytes/cytology ; Humans ; Inhibitor of Apoptosis Proteins/biosynthesis ; Inhibitor of Apoptosis Proteins/physiology ; Middle Aged ; Osteoarthritis/metabolism ; Osteoarthritis/pathology ; Protein Biosynthesis/genetics ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Survivin ; Transcriptional Activation/physiology

Background: Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes in vitro.
Methods: Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions in vitro were investigated by transfection of a specific siRNA.
Results: Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli in vitro.
Conclusions: The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. In vitro experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.

ScientificWorldJournal. 2010 Jun 29;10:1244-57. (PMID: 20602082)
Science. 2010 Oct 8;330(6001):235-9. (PMID: 20705815)
Br J Dermatol. 2006 Dec;155(6):1191-6. (PMID: 17107388)
Am J Sports Med. 2009 Jul;37(7):1324-33. (PMID: 19448050)
Inflammation. 2010 Dec;33(6):381-8. (PMID: 20195727)
Biochem Biophys Res Commun. 2005 Mar 18;328(3):658-65. (PMID: 15694399)
Arthritis Res Ther. 2005;7(2):R349-58. (PMID: 15743483)
Rheumatol Int. 2012 Jun;32(6):1541-8. (PMID: 21327438)
Am J Pathol. 2010 Dec;177(6):2816-26. (PMID: 21057000)
J Biol Chem. 2008 Jan 4;283(1):648-659. (PMID: 17993464)
Arthritis Rheum. 2002 Jun;46(6):1528-35. (PMID: 12115183)
Blood. 2011 Jan 6;117(1):352-61. (PMID: 20930069)
Science. 1996 Aug 2;273(5275):663-6. (PMID: 8662561)
Am J Pathol. 2007 Nov;171(5):1483-98. (PMID: 17982126)
Biochem J. 2010 Sep 1;430(2):199-205. (PMID: 20704571)
Ann N Y Acad Sci. 2009 Aug;1171:59-76. (PMID: 19723038)
Ann Med. 2010;42(1):45-54. (PMID: 20092399)
BMC Cancer. 2011 Apr 02;11:120. (PMID: 21457573)
Curr Biol. 2000 Nov 2;10(21):1319-28. (PMID: 11084331)
Nature. 1998 Dec 10;396(6711):580-4. (PMID: 9859993)
Osteoarthritis Cartilage. 2002 Aug;10(8):631-7. (PMID: 12479385)
Arthritis Rheum. 2010 May;62(5):1372-82. (PMID: 20213810)
Histol Histopathol. 2011 Mar;26(3):377-94. (PMID: 21210351)
J Cell Biol. 2000 Dec 25;151(7):1575-82. (PMID: 11134084)
Anal Biochem. 2000 Nov 1;286(1):80-5. (PMID: 11038277)
Clin Exp Rheumatol. 2005 Jul-Aug;23(4):550. (PMID: 16095130)
Osteoarthritis Cartilage. 2000 Jul;8(4):294-302. (PMID: 10903884)
Scand J Immunol. 2007 Aug-Sep;66(2-3):192-8. (PMID: 17635796)
Mol Cancer Ther. 2006 May;5(5):1087-98. (PMID: 16731740)
Am J Pathol. 2006 Jul;169(1):1-11. (PMID: 16816356)
Arthritis Res Ther. 2009;11(1):R13. (PMID: 19171073)
Curr Drug Targets. 2007 Feb;8(2):333-45. (PMID: 17305511)
J Orthop Res. 2000 May;18(3):356-63. (PMID: 10937621)
J Bone Joint Surg Am. 1971 Apr;53(3):523-37. (PMID: 5580011)
Nat Rev Cancer. 2008 Jan;8(1):61-70. (PMID: 18075512)
FEBS J. 2009 Dec;276(24):7375-85. (PMID: 19922475)
Osteoarthritis Cartilage. 2002 Jan;10(1):62-70. (PMID: 11795984)
Cancer Res. 2004 Oct 15;64(20):7183-90. (PMID: 15492230)
J Immunol Methods. 1999 Mar 4;223(2):185-94. (PMID: 10089097)
J Cell Mol Med. 2009 Sep;13(9B):3797-808. (PMID: 19298527)

0 (BIRC5 protein, human)
0 (Inhibitor of Apoptosis Proteins)
0 (RNA, Messenger)
0 (Survivin)

Date Created: 20110707 Date Completed: 20120419 Latest Revision: 20211020

20240628

PMC3141611

10.1186/1471-2474-12-150

21729321