Influence of water/O₂ plasma treatment on cellular responses of PCL and PET surfaces.

Publisher: IOS Press Country of Publication: Netherlands NLM ID: 9104021 Publication Model: Print Cited Medium: Internet ISSN: 1878-3619 (Electronic) Linking ISSN: 09592989 NLM ISO Abbreviation: Biomed Mater Eng Subsets: MEDLINE

Publication: Amsterdam : IOS Press
Original Publication: New York : Pergamon Press, c1991-

Biocompatible Materials/*chemistry ; Fibroblasts/*cytology ; Oxygen/*chemistry ; Oxygen/*pharmacology ; Polyesters/*chemistry ; Polyethylene Glycols/*chemistry ; Water/*chemistry; Cell Adhesion/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Fibroblasts/metabolism ; Fibroblasts/ultrastructure ; Gases/chemistry ; Humans ; Hydroxides/chemistry ; Membranes, Artificial ; Microscopy, Atomic Force/methods ; Microscopy, Electron, Scanning/methods ; Polyethylene Terephthalates ; Surface Properties

In this study, low pressure water/O₂ plasma treatment was performed in order to obtain COOH functionalities on the surface of poly-ε-caprolactone (PCL) membranes as well as non-woven polyester fabric (NWPF) discs. The plasma treatments were performed in a cylindrical, capacitively coupled RF-plasma-reactor and then following steps were performed: in situ (oxalyl chloride vapors) gas/solid reaction to convert -OH functionalities into -COCl groups; and hydrolysis under open laboratory conditions using air moisture for final-COOH functionalities. COOH and OH functionalities on modified surfaces were detected quantitatively by using fluorescent labeling technique and an UVX 300G sensor. Electron spectroscopy for chemical analysis (ESCA) was used to evaluate the relative surface atomic compositions and the carbon and oxygen linkages located in non-equivalent atomic positions of untreated and modified surfaces. Atomic force microscope (AFM) analysis showed that nanoscale features of the PCL surfaces are dramatically changed during the surface treatments. Scanning electron microscopy (SEM) results indicated the changes in the relatively smooth appearance of the untreated NWPF discs after the plasma treatment. Periodontal ligament (PDL) fibroblasts were used in cell culture studies. Cell culture results showed that plasma treated PCL membranes and NWPF discs were favorable for the PDL cell spreading, growth and viability due to the presence of functional groups and/or nanotopographies on their surfaces.

Erratum in: Biomed Mater Eng. 2011;21(3):191.

0 (Biocompatible Materials)
0 (Gases)
0 (Hydroxides)
0 (Membranes, Artificial)
0 (Polyesters)
0 (Polyethylene Terephthalates)
059QF0KO0R (Water)
24980-41-4 (polycaprolactone)
3WJQ0SDW1A (Polyethylene Glycols)
9159UV381P (hydroxide ion)
S88TT14065 (Oxygen)

Date Created: 20110610 Date Completed: 20110928 Latest Revision: 20181201

20240829

10.3233/BME-2011-0662

21654068