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Subcellular calcium transients visualized by confocal microscopy in a voltage-clamped vertebrate neuron.

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  • المؤلفون: Hernández-Cruz A;Hernández-Cruz A; Sala F; Adams PR
  • المصدر:
    Science (New York, N.Y.) [Science] 1990 Feb 16; Vol. 247 (4944), pp. 858-62.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print Cited Medium: Print ISSN: 0036-8075 (Print) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
    • بيانات النشر:
      Publication: : Washington, DC : American Association for the Advancement of Science
      Original Publication: New York, N.Y. : [s.n.] 1880-
    • الموضوع:
      Calcium/*metabolism ; Calcium Channels/*physiology ; Neurons/*physiology; Animals ; Caffeine/pharmacology ; Cytosol/metabolism ; Fluorescent Dyes ; Ganglia, Sympathetic/drug effects ; Ganglia, Sympathetic/physiology ; In Vitro Techniques ; Kinetics ; Membrane Potentials ; Microscopy, Fluorescence/methods ; Neurons/cytology ; Neurons/drug effects ; Rana catesbeiana
    • نبذة مختصرة :
      Confocal laser-scanned microscopy and long-wavelength calcium (Ca2+) indicators were combined to monitor both sustained and rapidly dissipating Ca2+ gradients in voltage-clamped sympathetic neurons isolated from the bullfrog. After a brief activation of voltage-dependent Ca2+ channels, Ca2+ spreads inwardly, and reaches the center of these spherical cells in about 300 milliseconds. Although the Ca2+ redistribution in the bulk of the cytosol could be accounted for with a radial diffusion model, local nonlinearities, suggesting either nonuniform Ca2+ entry or spatial buffering, could be seen. After electrical stimulation, Ca2+ signals in the nucleus were consistently larger and decayed more slowly than those in the cytosol. A similar behavior was observed when release of intracellular Ca2+ was induced by caffeine, suggesting that in both cases large responses originate from Ca2+ release sites near or within the nucleus. These results are consistent with an amplification mechanism involving Ca2(+)-induced Ca2+ release, which could be relevant to activity-dependent, Ca2(+)-regulated nuclear events.
    • Grant Information:
      NS1857906 United States NS NINDS NIH HHS
    • الرقم المعرف:
      0 (Calcium Channels)
      0 (Fluorescent Dyes)
      3G6A5W338E (Caffeine)
      SY7Q814VUP (Calcium)
    • الموضوع:
      Date Created: 19900216 Date Completed: 19900320 Latest Revision: 20190618
    • الموضوع:
      20221208
    • الرقم المعرف:
      10.1126/science.2154851
    • الرقم المعرف:
      2154851