Odorant-binding proteins and chemosensory proteins in pheromone detection and release in the silkmoth Bombyx mori.

Publisher: Oxford University Press Country of Publication: England NLM ID: 8217190 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3553 (Electronic) Linking ISSN: 0379864X NLM ISO Abbreviation: Chem Senses Subsets: MEDLINE

Publication: Oxford : Oxford University Press
Original Publication: [London : IRL Press, c1980-

Bombyx/*metabolism ; Insect Proteins/*metabolism ; Pheromones/*metabolism ; Receptors, Odorant/*metabolism; Animals ; Arthropod Antennae/metabolism ; Bombyx/genetics ; Female ; Gene Expression ; Insect Proteins/genetics ; Male ; Proteomics ; Receptors, Odorant/genetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

The genome of the silkmoth Bombyx mori contains 44 genes encoding odorant-binding proteins (OBPs) and 20 encoding chemosensory proteins (CSPs). In this work, we used a proteomic approach to investigate the expression of proteins of both classes in the antennae of adults and in the female pheromone glands. The most abundant proteins found in the antennae were the 4 OBPs (PBP, GOBP1, GOBP2, and ABP) and the 2 CSPs (CSP1 and CSP2) previously identified and characterized. In addition, we could detect only 3 additional OBPs and 2 CSPs, with clearly different patterns of expression between the sexes. Particularly interesting, on the other hand, is the relatively large number of binding proteins (1 OBP and 7 CSPs) expressed in the female pheromone glands, some of them not present in the antennae. In the glands, these proteins could be likely involved in the solubilization of pheromonal components and their delivery in the environment.

0 (Insect Proteins)
0 (Pheromones)
0 (Receptors, Odorant)
0 (odorant-binding protein)

Date Created: 20110112 Date Completed: 20110729 Latest Revision: 20110412

20231215

10.1093/chemse/bjq137

21220518