Isolation, fine mapping and expression profiling of a lesion mimic genotype, spl(NF4050-8) that confers blast resistance in rice.

Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE

Original Publication: Berlin, New York, Springer

Gene Expression Profiling* ; Physical Chromosome Mapping*; Genes, Plant/*genetics ; Immunity, Innate/*genetics ; Magnaporthe/*physiology ; Oryza/*genetics ; Plant Diseases/*immunology; Alleles ; Chromosome Segregation/genetics ; Chromosomes, Plant/genetics ; Crosses, Genetic ; Gene Expression Regulation, Plant ; Gene Library ; Genetic Linkage ; Genetic Loci/genetics ; Genotype ; Inheritance Patterns/genetics ; Japan ; Molecular Sequence Annotation ; Mutagenesis, Insertional/genetics ; Mutation/genetics ; Nucleotides/genetics ; Oligonucleotide Array Sequence Analysis ; Oryza/immunology ; Oryza/microbiology ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Regeneration/genetics ; Tissue Culture Techniques

We evaluated a large collection of Tos17 mutant panel lines for their reaction to three different races of Magnaporthe oryzae and identified a lesion mimic mutant, NF4050-8, that showed lesions similar to naturally occurring spl5 mutant and enhanced resistance to all the three blast races tested. Nested modified-AFLP using Tos17-specific primers and southern hybridization experiments of segregating individuals indicated that the lesion mimic phenotype in NF4050-8 is most likely due to a nucleotide change acquired during the culturing process and not due to Tos17 insertion per se. Inheritance and genetic analyses in two japonica × indica populations identified an overlapping genomic region of 13 cM on short arm of chromosome 7 that was linked with the lesion mimic phenotype. High-resolution genetic mapping using 950 F(3) and 3,821 F(4) plants of NF4050-8 × CO39 delimited a 35 kb region flanked by NBARC1 (5.262 Mb) and RM8262 (5.297 Mb), which contained 6 ORFs; 3 of them were 'resistance gene related' with typical NBS-LRR signatures. One of them harbored a NB-ARC domain, which had been previously demonstrated to be associated with cell death in animals. Microarray analysis of NF4050-8 revealed significant up-regulation of numerous defense/pathogenesis-related genes and down-regulation of heme peroxidase genes. Real-time PCR analysis of WRKY45 and PR1b genes suggested possible constitutive activation of a defense signaling pathway downstream of salicylic acid but independent of NH1 in these mutant lines of rice.

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Date Created: 20101207 Date Completed: 20110613 Latest Revision: 20211020

20231215

10.1007/s00122-010-1490-7

21132425