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Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus.

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  • المؤلفون: Zhang K;Zhang K; Wang L; Liu M; Zhang R; Li J
  • المصدر:
    Virology journal [Virol J] 2010 May 20; Vol. 7, pp. 101. Date of Electronic Publication: 2010 May 20.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X (Electronic) Linking ISSN: 1743422X NLM ISO Abbreviation: Virol J Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: [London] : BioMed Central, 2004-
    • الموضوع:
      Enzyme-Linked Immunosorbent Assay/*standards ; Hepatitis Antibodies/*analysis ; Hepatitis E/*blood ; Immunoglobulin M/*analysis; Animals ; Hepatitis Antibodies/chemistry ; Hepatitis E/immunology ; Hepatitis E virus/immunology ; Humans ; Immunoglobulin G/analysis ; Immunoglobulin G/chemistry ; Immunoglobulin M/chemistry ; Rabbits ; Reference Standards
    • نبذة مختصرة :
      Background: In assays for anti-hepatitis E virus (HEV) immunoglobulin M (IgM), large volumes of the patient's sera cannot be easily obtained for use as a positive control. In this study, we investigated an alternative chemical method in which rabbit anti-HEV IgG was conjugated with human IgM and was used as a positive control in the anti-HEV IgM assay. Rabbit anti-HEV IgG was isolated from immune sera by chromatography on protein A-Sepharose and was conjugated with human IgM by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinker.
      Results: The specific anti-HEV IgG antibody titer was 100,000 times that of the negative control, i.e., prebleed rabbit serum. The results of anti-HEV IgM enzyme-linked immunosobent assay showed that the antibody conjugate was similar to anti-HEV IgM antibodies produced in humans. The results of a stability experiment showed that the antibody conjugate was stable for use in external quality assessment or internal quality control trials.
      Conclusions: We concluded that the chemically conjugated rabbit-human antibody could be used instead of the traditional serum control as a positive control in the anti-HEV IgM assay.
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    • الرقم المعرف:
      0 (Hepatitis Antibodies)
      0 (Immunoglobulin G)
      0 (Immunoglobulin M)
    • الموضوع:
      Date Created: 20100521 Date Completed: 20100907 Latest Revision: 20211020
    • الموضوع:
      20221213
    • الرقم المعرف:
      PMC2881894
    • الرقم المعرف:
      10.1186/1743-422X-7-101
    • الرقم المعرف:
      20482892