Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Promoter Regions, Genetic*; Myelin Proteins/*genetics ; Xenopus Proteins/*genetics ; Zebrafish/*genetics; Alternative Splicing ; Animals ; Animals, Genetically Modified ; Cell Line ; Embryo, Nonmammalian/metabolism ; GATA Transcription Factors/metabolism ; Intestinal Mucosa/metabolism ; Liver/metabolism ; Mice ; Myelin Proteins/metabolism ; Nogo Proteins ; Xenopus Proteins/metabolism ; Zebrafish/embryology ; Zebrafish/metabolism ; Zebrafish Proteins

In mammals, the Nogo family consists of Nogo-A, Nogo-B and Nogo-C. However, there are three Rtn-4/Nogo-related transcripts were identified in zebrafish. In addition to the common C-terminal region, the N-terminal regions of Rtn4-n/Nogo-C1, Rtn4-m/Nogo-C2 and Rtn4-l/Nogo-B, respectively, contain 9, 25 and 132 amino acid residues. In this study, we isolated the 5'-upstream region of each gene from a BAC clone and demonstrated that the putative promoter regions, P1-P3, are functional in cultured cells and zebrafish embryos. A transgenic zebrafish Tg(Nogo-B:GFP) line was generated using P1 promoter region to drive green fluorescent protein (GFP) expression through Tol2-mediated transgenesis. This line recapitulates the endogenous expression pattern of Rtn4-l/Nogo-B mRNA in the brain, brachial arches, eyes, muscle, liver and intestines. In contrast, GFP expressions by P2 and P3 promoters were localized to skeletal muscles of zebrafish embryos. Several GATA and E-box motifs are found in these promoter regions. Using morpholino knockdown experiments, GATA4 and GATA6 were involved in the control of P1 promoter activity in the liver and intestine, while Myf5 and MyoD for the control of P1 and P3 promoter activities in muscles. These data demonstrate that zebrafish Rtn4/Nogo transcripts might be generated by coupling mechanisms of alternative first exons and alternative promoter usage.

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0 (GATA Transcription Factors)
0 (Myelin Proteins)
0 (Nogo Proteins)
0 (Rtn4 protein, mouse)
0 (Rtn4a protein, zebrafish)
0 (Xenopus Proteins)
0 (Zebrafish Proteins)

Date Created: 20100410 Date Completed: 20100923 Latest Revision: 20211020

20240829

PMC2919723

10.1093/nar/gkq230

20378713