15-Deoxy-delta 12, 14,-prostaglandin J2 suppresses nuclear factor-kappaB-mediated apoptosis of Helicobacter pylori-infected gastric epithelial cells.

Publisher: New York Academy of Sciences Country of Publication: United States NLM ID: 7506858 Publication Model: Print Cited Medium: Internet ISSN: 1749-6632 (Electronic) Linking ISSN: 00778923 NLM ISO Abbreviation: Ann N Y Acad Sci Subsets: MEDLINE

Publication: 2006- : New York, NY : Malden, MA : New York Academy of Sciences ; Blackwell
Original Publication: New York, The Academy.

Apoptosis/*drug effects ; Helicobacter pylori/*growth & development ; NF-kappa B/*metabolism; Adenocarcinoma/metabolism ; Adenocarcinoma/microbiology ; Adenocarcinoma/pathology ; Antigens, Bacterial/genetics ; Bacterial Proteins/genetics ; Blotting, Western ; Cell Line, Tumor ; DNA Fragmentation/drug effects ; Electrophoretic Mobility Shift Assay ; Enzyme-Linked Immunosorbent Assay/methods ; Helicobacter pylori/genetics ; Humans ; Oligonucleotides/genetics ; Oligonucleotides/metabolism ; Prostaglandin D2/analogs & derivatives ; Prostaglandin D2/pharmacology ; Protein Binding/drug effects ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/microbiology ; Stomach Neoplasms/pathology ; bcl-2-Associated X Protein/metabolism

Helicobacter pylori deregulates the genes that control homeostasis between apoptosis and cell proliferation of gastric epithelial cells. Nuclear factor-kappaB (NF-kappaB) has an important role in H. pylori-induced apoptosis in gastric epithelial cells. The peroxisome proliferator-activated receptor-gamma ligand 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) regulates growth and the signaling cascade in H. pylori-infected gastric epithelial cells. In the present study, we determined whether 15d-PGJ(2) inhibits apoptosis by regulating apoptotic gene expression and NF-kappaB activation in gastric epithelial cells infected with CagA+, VacA+H. pylori in a Korean isolate (HP99). 15d-PGJ(2) was found to inhibit H. pylori-induced DNA fragmentation and cell death. 15d-PGJ(2) induced downregulation of proapoptotic Bax and upregulation of antiapoptotic Bcl-2 as well as suppression of NF-kappaB activation caused by H. pylori in gastric epithelial cells. The results suggest that 15d-PGJ(2) inhibits apoptotic cell death by inhibiting NF-kappaB activation and apoptotic gene expression in gastric epithelial cells.

0 (15-deoxy-delta(12,14)-prostaglandin J2)
0 (Antigens, Bacterial)
0 (Bacterial Proteins)
0 (NF-kappa B)
0 (Oligonucleotides)
0 (Proto-Oncogene Proteins c-bcl-2)
0 (VacA protein, Helicobacter pylori)
0 (bcl-2-Associated X Protein)
0 (cagA protein, Helicobacter pylori)
RXY07S6CZ2 (Prostaglandin D2)

Date Created: 20090903 Date Completed: 20090923 Latest Revision: 20131121

20221213

10.1111/j.1749-6632.2009.04706.x

19723089