Determination of yessotoxins and pectenotoxins in shellfish by capillary electrophoresis-electrospray ionization-mass spectrometry.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 101485040 Publication Model: Print Cited Medium: Internet ISSN: 1944-0057 (Electronic) Linking ISSN: 19440057 NLM ISO Abbreviation: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: Abingdon, Oxon ; Philadelphia, PA : Taylor & Francis, 2008-

Marine Toxins/*analysis ; Oxocins/*analysis ; Shellfish/*analysis ; Spectrometry, Mass, Electrospray Ionization/*methods; Animals ; Furans/analysis ; Furans/chemistry ; Macrolides ; Marine Toxins/chemistry ; Mollusk Venoms ; Oxocins/chemistry ; Pyrans/analysis ; Pyrans/chemistry ; Sensitivity and Specificity ; Spain

Conditions for the determination of lipophilic marine toxins, such as yessotoxins and pectenotoxins (PTX)-6, were investigated with capillary electrophoresis coupled to mass spectrometry (MS) with an electrospray ionization source. After optimization, a simple and MS compatible alkaline volatile buffer solution of ammonium acetate was selected as background electrolyte, with isopropanol/water (80/20, v/v) sheath liquid modified with ammonium acetate used at the electrospray ionization (ESI) source. Previously to capillary electrophoresis (CE) separations, the application of an on-line sample pre-concentration approach based on field-amplified sample stacking was accomplished to increase sensitivity. As a result, the limits of detection provided by capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) were 0.02 microg ml(-1) (0.01 microg g(-1)), which corresponded to 1.25 pg for yessotoxin and 0.25 microg ml(-1) (0.13 microg g(-1) and 13.25 pg on capillary) for PTX-6. Accuracy tests showed 97.7% recovery from spiked blank mussel samples that showed no significant matrix influence running under optimal conditions. Intermediate precision was close to 4% relative standard deviation (RSD) for the migration time, and an RSD of 7.5% for peak areas. The method was successfully applied to naturally contaminated seafood samples in which yessotoxins and pectenotoxins-6 were clearly determined. This work demonstrated the potential of CE-ESI-MS to be applied for a sensitive determination of lipophilic toxins from the marine environment as alternative to liquid chromatography-electrospray ionization-single quadrupole mass spectrometry (LC-ESI-MS) for this purpose.

0 (Furans)
0 (Macrolides)
0 (Marine Toxins)
0 (Mollusk Venoms)
0 (Oxocins)
0 (Pyrans)
0 (pectenotoxin 6)
54NE3O7Z3A (pectenotoxin 1)
97564-91-5 (pectenotoxin 2)
P6M9FM2L2G (yessotoxin)

Date Created: 20090815 Date Completed: 20100514 Latest Revision: 20211203

20240829

10.1080/02652030802290522

19680893