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Effect of rhBMP-7 combined with two bone grafts on human periodontal ligament cell differentiation.

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  • المؤلفون: Dereka XE;Dereka XE; Markopoulou CE; Mamalis A; Vrotsos IA
  • المصدر:
    Growth factors (Chur, Switzerland) [Growth Factors] 2009 Oct; Vol. 27 (5), pp. 274-9.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 9000468 Publication Model: Print Cited Medium: Internet ISSN: 1029-2292 (Electronic) Linking ISSN: 08977194 NLM ISO Abbreviation: Growth Factors Subsets: MEDLINE
    • بيانات النشر:
      Publication: London : Informa Healthcare
      Original Publication: Chur ; New York : Harwood Academic Publishers, 1988-
    • الموضوع:
      Bone Morphogenetic Protein 7/*pharmacology ; Bone Transplantation/*methods ; Cell Differentiation/*drug effects ; Osteoblasts/*drug effects ; Periodontal Ligament/*cytology ; Periodontal Ligament/*drug effects ; Recombinant Proteins/*pharmacology; Adult ; Animals ; Bone Morphogenetic Protein 7/genetics ; Bone Substitutes/therapeutic use ; Cattle ; Cells, Cultured ; Female ; Humans ; Male ; Osteoblasts/cytology ; Recombinant Proteins/genetics ; Treatment Outcome ; Young Adult
    • نبذة مختصرة :
      The purpose of this study was to evaluate the in vitro effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) combined with demineralised freeze-dried bone allograft (DFDBA) and an inorganic bovine material with a synthetic peptide (PepGen P-15) on human periodontal ligament (hPDL) cell differentiation, in a time-dependent manner. hPDL cells were cultured and treated with: (1) 500 ng/ml of rhBMP-7, (2) 10 mg of DFDBA or PepGen P-15 and (3) their combination. Cell differentiation was estimated after 48 and 72 h by measuring alkaline phosphatase (ALPase) activity and osteocalcin (OC) secretion. The presence of rhBMP-7, DFDBA, PepGen P-15, rhBMP-7 + DFDBA and rhBMP-7+ PepGen P-15 promoted a significant increase of ALPase activity after 48 and 72 h. The combination of rhBMP-7 with DFDBA or PepGen P-15 did not lead to significant OC secretion. The results of this study imply that rhBMP-7 stimulates the early osteoblastic differentiation of hPDL cells and that DFDBA and PepGen P-15 could serve as carriers for rhBMP-7.
    • الرقم المعرف:
      0 (Bone Morphogenetic Protein 7)
      0 (Bone Substitutes)
      0 (PepGen P-15)
      0 (Recombinant Proteins)
    • الموضوع:
      Date Created: 20090717 Date Completed: 20091209 Latest Revision: 20220316
    • الموضوع:
      20250114
    • الرقم المعرف:
      10.1080/08977190903112721
    • الرقم المعرف:
      19606370