An integrated genetic and cytogenetic map of the cucumber genome.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Genome, Plant*; Chromosome Mapping/*methods ; Cucumis sativus/*genetics; Citrullus/genetics ; Cloning, Molecular ; Cucurbita/genetics ; Cucurbitaceae/genetics ; Cytogenetic Analysis ; DNA, Plant ; Genes, Plant ; Genetic Linkage ; Genetic Markers/genetics ; In Situ Hybridization, Fluorescence ; Polymorphism, Genetic ; Species Specificity

The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the development of highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and the subsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups which spans in total 573 cM, and defines approximately 680 recombination breakpoints with an average of 0.58 cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% were conserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positional cloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits.

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0 (DNA, Plant)
0 (Genetic Markers)

Date Created: 20090605 Date Completed: 20091117 Latest Revision: 20240326

20240326

PMC2685989

10.1371/journal.pone.0005795

19495411