Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum.

Publisher: Oxford University Press Country of Publication: Germany NLM ID: 9705544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5535 (Electronic) Linking ISSN: 13675435 NLM ISO Abbreviation: J Ind Microbiol Biotechnol Subsets: MEDLINE

Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Published by Stockton Press on behalf of the Society for Industrial Microbiology, c1996-

Industrial Waste* ; Penaeidae*; Food-Processing Industry/*methods ; Gram-Positive Bacteria/*metabolism ; Industrial Microbiology/*methods; Animals ; Bacillus cereus/metabolism ; Minerals/metabolism ; Proteins/metabolism ; Temperature ; Time Factors

Two bacterial cultures were isolated and tested for degradation of shrimp shell waste. According to morphological examination, physiological tests, and applied molecular techniques, isolates were identified as Bacillus cereus and Exiguobacterium acetylicum. Both strains were cultivated separately in flasks with 100 mL of shrimp shell waste broth (3% of washed, dried and ground shrimp shell waste in tap water, pH 7.0) at 37 degrees C. At determined periods of time, deproteinization and demineralization of residuals were measured. Fermentation of 3% shell waste with B. cereus indicated 97.1% deproteinization and 95% demineralization. For E. acetylicum, the level of deproteinization and demineralization was 92.8 and 92%, respectively. Protein content was reduced from 18.7 to 5.3% with B. cereus and to 7.3% with E. acetylicum. No additional supplements were used during the fermentation of shell waste. B. cereus strain showed higher efficacy in decomposition of shell waste and was used for large-scale fermentation in 12 L of 10% shrimp shell waste broth. Incubation of bacteria with shell waste during 14 days at 37 degrees C resulted in 78.6% deproteinization and 73% demineralization. High activity of isolated cultures in decomposition of shrimp shell waste suggests broad potential for application of these bacteria in environmentally friendly approaches to chitin extraction from chitin-rich wastes.

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0 (Industrial Waste)
0 (Minerals)
0 (Proteins)

Date Created: 20090528 Date Completed: 20090914 Latest Revision: 20211020

20250114

10.1007/s10295-009-0587-y

19471983