Vaccination with Streptococcus suis serotype 2 recombinant 6PGD protein provides protection against S. suis infection in swine.

Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1574-6968 (Electronic) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE

Publication: 2015- : Oxford Oxford University Press
Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.

Glucosephosphate Dehydrogenase/*immunology ; Streptococcal Infections/*veterinary ; Streptococcal Vaccines/*immunology ; Streptococcus suis/*immunology ; Swine Diseases/*prevention & control; Animals ; Antibodies, Bacterial/blood ; Bacterial Proteins/genetics ; Bacterial Proteins/immunology ; Brain/microbiology ; Brain/pathology ; Glucosephosphate Dehydrogenase/genetics ; Immunoglobulin G/blood ; Lung/microbiology ; Lung/pathology ; Streptococcal Infections/immunology ; Streptococcal Infections/prevention & control ; Streptococcal Vaccines/administration & dosage ; Survival Analysis ; Swine ; Swine Diseases/immunology ; Vaccines, Subunit/genetics ; Vaccines, Subunit/immunology ; Vaccines, Synthetic/genetics ; Vaccines, Synthetic/immunology

Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immunization with an r6PGD-containing adjuvant induced a vigorous immunoglobulin G (IgG) response, with the titer of IgG2 being higher than that of IgG1. Immunization of piglets with r6PGD yielded 50% survival upon an intravenous challenge with a lethal dose of SS2. Piglets immunized with the r6PDG vaccine were better protected than those immunized with the adjuvant control. The clinical signs and histopathological changes in the piglets were recorded. Collectively, these results suggest that r6PGD can confer partial protection against SS2 infection and could be useful for the development of subunit vaccines against SS2.

0 (Antibodies, Bacterial)
0 (Bacterial Proteins)
0 (Immunoglobulin G)
0 (Streptococcal Vaccines)
0 (Vaccines, Subunit)
0 (Vaccines, Synthetic)
EC 1.1.1.49 (Glucosephosphate Dehydrogenase)

Date Created: 20090523 Date Completed: 20090921 Latest Revision: 20090728

20240829

10.1111/j.1574-6968.2009.01617.x

19459970